Sequences from confirmation amplicon


Confirmation sequences for line 925B11 and hit At4g21560 are shown.
Line ID 925B11
Locus At4g21560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11469095
Primers used for PCR 10ek - 8474, show primer details
   
Sequence Name 30-K110713-0022-925-B11C-10ek
GenBank Accession LN499770 [GenBank]
Sequencing primer gene specific primer, 10ek: 5'-TTTCTGAAAGATGGAGGTCAAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K110713-0022-925-B11C-10ek
GAGAATTTCGCAGAGCTTTATGCTATCATCAAAGCTACTGAGAAGCTCGAGAAGGCTTAT
ATTCGTGATCTAATCTCTCCATCTGAATACGAAACCGAGTGCCAGAAACTCATTGTTCAC
TTCAAGACATTATCTGCATCTCTCAAAGATATGGTCCCAAACATTGAGAGATTTGCAGAG
ACGTACAAGATGGATTGCTCAGCTGCAGTTTATCGCCTTGTGACTTCTGGTGTTCCAGCT
ACTGTGGAGCATAGAGCTGCTGCTTCGGCTTCTACATCAAGCTCTGCTTCTGTTGTTGCT
GAGTGTGTTCAGAACTTCATCACTTCCATGGATTCTTTGAAACTCAACATGGTTGCTGTT
GATCAGGTGTATCCGTTGTTGTCTGATCTCTCGGCTTCTCTTAACAAACTAAGTATATTG
CCACCAGATTTCGAGGGGAAGATTAAGATGAAGGAATGGCTTTTGAGGCTGTCAAAGATG
GGAGCTTCAGATGAACTCACTGAGCAGCAGGCTCGGCAACTTCACTTTGAGCATCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 14-K110711-0022-925-B11C-8409
GenBank Accession LN492560 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K110711-0022-925-B11C-8409
CGGAATGAGCATTTACATTGAATATATCCTGATGCTCAAGTGAAGTTGCCGAGCCTGCTG
CTCAGTGAGTTCATCTGAAGCTCCCATCTTTGACAGCCTCAAAAGCCATTCCTTCATCTT
AATCTTCCCCTCGAAATCTGGTGGCAATATACTTAGTTTGTTAAGAGAAGCCGAGAGATC
AGACAACAACGGATACACCTGATCAACAGCAACCATGTTGAGTTTCAAAGAATCCATGGA
AGTGATGAAGTTCTGAACACACTCAGCAACAACAGAAGCAGAGCTTGATGTAGAAGCCGA
AGCAGCAGCTCTATGCTCCACAGTAGCTGGAACACCAGAAGTCACAAGGCGATAAACTGC
AGCTGAGCAATCCATCTTGTACGTCTCTGCAAATCTCTCAATGTTTGGGACCATATCTTT
GAGAGATGCAGATAATGTCTTGAAGTGAACAATGAGTTTCTGGCACTCGGTTTCGTATTC
AGATGGAGAGATTAGATCACGAATATAAGCCTTCTCGAGCTTCTCAGTAGCTTTGATGAT
AGCATAAAGCTCTGCGAAATTCTCATACATTTCTCTCTCACGCTTATCATTCCATAACTT
GACCTCCATCTTTCAGAA

Last Updated on 06.12.2018 16:11