Sequences from confirmation amplicon


Confirmation sequences for line 925C04 and hit At1g52830 are shown.
Line ID 925C04
Locus At1g52830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19672701
Primers used for PCR 03rr - 8474, show primer details
   
Sequence Name 41-K116204-0022-925-C04C-03rr
GenBank Accession LN504211 [GenBank]
Sequencing primer gene specific primer, 03rr: 5'-AGTTGATTACCCTCGTTTCCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116204-0022-925-C04C-03rr
ATGAATCATTCAGACGAAAAAAAAAAGTATTGTGAGGCTAAGAAACACCTTTAATGAGCC
ATAAAACATTAAAGTATGATACGACCAGTAAGGAAATACATTTGAAGAAAACGTATATAT
CATCATTATCATATAAATTTTACAATTTGAAAAATGATTATAATGAAAAAAAATTACTCA
ACGGTTTTCAAACTTTATGGTTAATTAAAGATGATCAACACTAGCTAGTATGATTAATCT
TGCTGGAGACCAAAACCAGTTGCATGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 05-K116203-0022-925-C04C-8409
GenBank Accession LN488499 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116203-0022-925-C04C-8409
ATATCCATGCAACTGGGTTTTGGTCTCCAGCAAGATTAATCATACTAGCTAGTGTTGATC
ATCTTTAATTAACCATAAAGTTTGAAAACCGTTGAGTAATTTTTTTTCATTATAATCATT
TTTCAAATTGTAAAATTTATATGATAATGATGATATATACGTTTTCTTCAAATGTATTTC
CTTACTGGTCGTATCATACTTTAATGTTTTATGGCTCATTAAAGGTGTTTCTTAGCCTCA
CAATACTTTTTTTTTTCGTCTGAATGATTCATATGAAATATAACACTTGAAATTGTTCAC
AAAAGTACAAAAGGAAACGAGGTTAATCAA

Last Updated on 06.12.2018 16:11