Sequences from confirmation amplicon


Confirmation sequences for line 926A06 and hit At2g24000 are shown.
Line ID 926A06
Locus At2g24000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10210518
Primers used for PCR CD80 - 8474, show primer details
   
Sequence Name 65-K105568-0022-926-A06C-CD80
GenBank Accession LN511404 [GenBank]
Sequencing primer gene specific primer, CD80: 5'-CAAAGACACCAGAGAAGTTTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K105568-0022-926-A06C-CD80
AACATATAAAATCATTAGAATGTGTCACTAAGAAATATTACATTCTTAGTATAGCTTATG
TTTTTGCAATACGTAAAAATGAAAATAATTCTTATCAACACAAACATATTCCTAAATCCT
CGTTAAAAAACATATGTACCCTAGTGATTTGTGTTAGTGTAATTGTTATGTCATCACTTC
AATTCAAATCGGCGATGTACTGAAATAAAATCGTCATCAAATAAAAAATGGAAGACGTCA
AATCTCATTAACATGTTACATGATTATTCTTAAATAGACTAACTAAAAGTAAAATATCAA
AAATACCTATATCAACACTTTATGTTTTATCATTATTTAAAGCAATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCC
   
Sequence Name 81-K105569w-0022-926-A06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K105569w-0022-926-A06C-8409
GGTATTTTTTGATATTTTACTTTTAGTTAGTCTATTTAAGAATAATCATGTAACATGTTA
ATGAGATTTGACGTCTTCCATTTTTTATTTGATGACGATTTTATTTCAGTACATCGCCGA
TTTGAATTGAAGTGATGACATAACAATTACACTAACACAAATCACTAGGGTACATATGTT
TTTTAACGAGGATTTAGGAATATGTTTGTGTTGATAAGAATTATTTTCATTTTTACGTAT
TGCAAAAACATAAGCTATACTAAGAATGTAATATTTCTTAGTGACACATTCTAATGATTT
CTTATATTGTTTTAAATTCTATATATTTGGTATCACAAACTTCTCTGGGTGTCTTTG
Primers used for PCR 05mg - 8474, show primer details
   
Sequence Name 64-K118446-0022-926-A06C-05mg
GenBank Accession LN511295 [GenBank]
Sequencing primer gene specific primer, 05mg: 5'-CTTAGCTTAGTGAAAGTCCGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118446-0022-926-A06C-05mg
TTATTCGATAATATTTTGGAGAGATTTTCGGCACCTATGAACTCCGGCCACAACTCATCA
ATTTGCCAAAAACAATGAAAATAAAAATTACTTTCTCTATAATTGATAGTTATAGAGTAA
TTAAATAAAACATAAGAGACGTAACTATCAAAACAAAGACACCAGAGAAGTTTGTGATAC
CAAATATATAGAATTTAAAACAATATAAGAAATCATTAGAATGTGTCACTAAGAAATATT
ACATTCTTAGTATAGCTTATGTTTTTGCAATACGTAAAAATGAAAATAATTCTTATCAAC
ACAAACATATTCCTAAATCCTCGTTAAAAAACATATGTACCCTAGTGATTTGTGTTAGTG
TAATTGTTATGTCATCACTTCAATTCAAATCGGCGATGTACTGAAATAAAATCGTCATCA
AATAAAAAATGGAAGACGTCAAATCTCATTAACATGTTACATGATTATTCTTAAATAGAC
TAACTAAAAGTAAAATATCAAAAATACCTATATCAACACTTTATGTTTTATCATTATTTA
AAGCAATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 24-K118445-0022-926-A06C-8409
GenBank Accession LN497190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118445-0022-926-A06C-8409
TTGATATAGGTATTTTTGATATTTTACTTTTAGTTAGTCTATTTAAGAATAATCATGTAA
CATGTTAATGAGATTTGACGTCTTCCATTTTTTATTTGATGACGATTTTATTTCAGTACA
TCGCCGATTTGAATTGAAGTGATGACATAACAATTACACTAACACAAATCACTAGGGTAC
ATATGTTTTTTAACGAGGATTTAGGAATATGTTTGTGTTGATAAGAATTATTTTCATTTT
TACGTATTGCAAAAACATAAGCTATACTAAGAATGTAATATTTCTTAGTGACACATTCTA
ATGATTTCTTATATTGTTTTAAATTCTATATATTTGGTATCACAAACTTCTCTGGTGTCT
TTGTTTTGATAGTTACGTCTCTTATGTTTTATTTAATTACTCTATAACTATCAATTATAG
AGAAAGTAATTTTTATTTTCATTGTTTTTGGCAAATTGATGAGTTGTGGCCGGAGTTCAT
AGGTGCCGAAAATCTTTCCAAAATATTATCGAATTAAGGACACAAGGTTCTTTTTGACCG
ATAGCTTCCGGACTTT

Last Updated on 06.12.2018 16:11