Sequences from confirmation amplicon


Confirmation sequences for line 928G02 and hit At1g57870 are shown.
Line ID 928G02
Locus At1g57870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21431523
Primers used for PCR 59tv - 8474, show primer details
   
Sequence Name 21-K117167-0022-928-G02C-59tv
GenBank Accession LN495832 [GenBank]
Sequencing primer gene specific primer, 59tv: 5'-GACTTACGAGTGTAATGCCATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117167-0022-928-G02C-59tv
GATACCAGAGAGTTCTGTAGGACACACNGCTTATCAATAACACATACTAATTTTCTTCCC
TTATAAAGAATTTAACACGGAAAAAAGAAGAAAGAAATGAAAAACGTAGATGGCTTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCG
   
Sequence Name 10-K117166-0022-928-G02C-8409
GenBank Accession LN490837 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117166-0022-928-G02C-8409
TGAAGCCATCTACGTTTTTCATTTCTTTCTTCTTTTTTCCGTGTTAAATTCTTTATAAGG
GAAGAAAATTAGTATGTGTTATTGATAAGCGTGTGTCCTACAGAACTCTCTGGTATCCCT
CCTGAAACTGTGGACCGACTTGTCCCAGAACATGCCCGTAAGCAGAACCACTTCATGGCA
TTACACTCGTAAGTC
Primers used for PCR 31ut - 8474, show primer details
   
Sequence Name 64-K117280-0022-928-G02C-31ut
GenBank Accession LN511284 [GenBank]
Sequencing primer gene specific primer, 31ut: 5'-TTTCCTCAGATAAAACCTCATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K117280-0022-928-G02C-31ut
CTAATAGGGTTCTCTTAATCGATATATGACCAAGCTTGTCTCGGTTTAGTGAGGTTGATT
GAAGTATATGTGATCAATTAACTTCATATTTCATTCCTCTGGCATATAGAGCGATGTGAC
AGCTTTGCCTTTGTATATAAGATTCTTATACAAACAAATAAATTGAACCTTTGCCAAAAG
GAATTACTCATGCACACACTCGTGGGGGTTGAAAACAGTTAGGCTTCATGATCTTGGCTT
TTTTGCTGGTTTAAGTTTCTATCTAGATGAAGTTTTATTCAGCATTTTATACAGCTGACA
TTCCAGAACTCTGTTACTCGTTATTCTTATCTTTTGTTTTGCTCCGTGCTAAAATTTCTC
TTAAGTGATCATTTCCTTGGTTTTACTTCTATATAGTCATGTTCTTACAGCACAAGAATC
ATGTGTTTGTTACTTTCAGGTATTCCAGAAACGTCTGCCACCTGAAGCAGTAGATCTTCT
TTGTAGGTTCTTCCAGTATTCTCCTAATTTGAGATGTACAGCGGTAAATTATTCATAGCT
TTTCTCTGACTTTGTTACATATTCTACTTCCATATTTTTTTTGGGATCAGCATCAGAAGC
TAACCCATTATAAGTATGTCTTATGATTTTCTGATAGGTGGAAGCTTGTATCCACCCTTT
CTTTGATGAGCTAAGAGACCCAAATGCTCGTCTGCCAAACGGCCGGCCACTTCCTCCGCT
ATTCAATTTTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCG
   
Sequence Name 40-K117279-0022-928-G02C-8409
GenBank Accession LN503847 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117279-0022-928-G02C-8409
CATTAAAATTGATAGCGGAGGAAGTGGCCGGCCGTTTGGCAGACGAGCATTTGGGTCTCT
TAGCTCATCAAAGAAAGGGTGGATACAAGCTTCCACCTATCAGAAAATCATAAGACATAC
TTATAATGGGTTAGCTTCTGATGCTGATCCCAAAAAAAATATGGAAGTAGAATATGTAAC
AAAGTCAGAGAAAAGCTATGAATAATTTACCGCTGTACATCTCAAATTAGGAGAATACTG
GAAGAACCTACAAAGAAGATCTACTGCTTCAGGTGGCAGACGTTTCTGGAATACCTGAAA
GTAACAAACACATGATTCTTGTGCTGTAAGAACATGACTATATAGAAGTAAAACCAAGGA
AATGATCACTTAAGAGAAATTTTAGCACGGAGCAAAACAAAAGATAAGAATAACGAGTAA
CAGAGTTCTGGAATGTCAGCTGTATAAAATGCTGAATAAAACTTCATCTAGATAGAAACT
TAAACCAGCAAAAAAGCCAAGATCATGAAGCCTAACTGTTTTCAACCCCCACGAGTGTGT
GCATGAGTAATTCCTTTTGGCAAAGGTTCAATTTATTTGTTTGTATAAGAATCTTATATA
CAAAGGCAAAGCTGTCACATCGCTCTATATGCCAGAGGAATGAAATATGAAGTTAATTGA
TCACATATACTTCAATCAACCTCACTAAACCGAGACAAGCTTGGTCATATATCGATTAAG
AGAACCCTATTAGGTGATAATTGATACTACTAGCGGTATACCTTGTGCCAAGGATGAGGT
T
Primers used for PCR 30ut - 8474, show primer details
   
Sequence Name 65-K117280-0022-928-G02C-30ut
GenBank Accession LN511479 [GenBank]
Sequencing primer gene specific primer, 30ut: 5'-TTCAAATCAAGTTCGTAAACATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K117280-0022-928-G02C-30ut
TATTTTAGGTTACAATTTTTTGGAAATTGTGTTGTGGCAGGAGTCTTGCAAATGTAAGTA
TGTTCCTCTATATCATATATCTAACAAAGTAGTGTTGTATTTTACGCATCCTCACTTCAC
GACAAGGGATGCGCAAGCACCTTAATACAAAAGTCATTGCTTCTTTTTCCAGTGATTTCT
GTTTACAGAGAGAGAGCTCTACTGGTTTTTCCCAAAGACTGGGATCTAGAGAGCTTAGAG
AAAAGAAGAGAACACCCGATAGGTCGTACAGAAAACACGGAAGAGACTTACGAGTGTAAT
GCCATGAAGTGGTTCTGCTTACGGGCATGTTCTGGGACAAGTCGGTCCACAGTTTCAGGA
GGGATACCAGAGAGTTCTGTAGGACACACGCTTATCAATAACACATACTAATTTTCTTCC
CTTATAAAGAATTTAACACGGAAAAAAGAAGAAAGAAATGAAAAACGTAGATGGCTTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
A
   
Sequence Name 41-K117279-0022-928-G02C-oriL
GenBank Accession LN504232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117279-0022-928-G02C-oriL
TTGAAGCCATCTACGTTTTTCATTTCTTTCTTCTTTTTTCCGTGTTAAATTCTTTATAAG
GGAAGAAAATTAGTATGTGTTATTGATAAGCGTGTGTCCTACAGAACTCTCTGGTATCCC
TCCTGAAACTGTGGACCGACTTGTCCCAGAACATGCCCGTAAGCAGAACCACTTCATGGC
ATTACACTCGTAAGTCTCTTCCGTGTTTTCTGTACGACCTATCGGGTGTTCTCTTCTTTT
CTCTAAGCTCTCTAGATCCCAGTCTTTGGGAAAAACCAGTAGAGCTCTCTCTCTGTAAAC
AGAAATCACTGGAAAAAGAAGCAATGACTTTTGTATTAAGGTGCTTGCGCATCCCTTGTC
GTGAAGTGAGGATGCGTAAAATACAACACTACTTTGTTAGATATATGATATAGAGGAACA
TACTTACATTTGCAAGACTCCTGCCACAACACAATTTTCCAAAAAATTTGTGAACCTTAA
AATGAATTGACAGTTTAGTTTATTTGTCCATGTTTACGAATGGAAATTTG
Primers used for PCR 63vm - 8474, show primer details
   
Sequence Name 38-K117776-0022-928-G02C-63vm
GenBank Accession LN503137 [GenBank]
Sequencing primer gene specific primer, 63vm: 5'-TTTTGTGTACATGGTCTTATTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K117776-0022-928-G02C-63vm
AACAATGCCTTATTTGAATTAACTCGAAGATAAATTGGTTTGGTGTAATTCGAGTAATAT
GGTTCAAATCAAGTTCGTAAACATGGACAAATAAACTAAACTGTCAATTCATTTTAAGGT
TCACAAATTTTTTGGAAAATTGTGTTGTGGCAGGAGTCTTGCAAATGTAAGTATGTTCCT
CTATATCATATATCTAACAAAGTAGTGTTGTATTTTACGCATCCTCACTTCACGACAAGG
GATGCGCAAGCACCTTAATACAAAAGTCATTGCTTCTTTTTCCAGTGATTTCTGTTTACA
GAGAGAGAGCTCTACTGGTTTTTCCCAAAGACTGGGATCTAGAGAGCTTAGAGAAAAGAA
GAGAACACCCGATAGGTCGTACAGAAAACACGGAAGAGACTTACGAGTGTAATGCCATGA
AGTGGTTCTGCTTACGGGCATGTTCTGGGACAAGTCGGTCCACAGTTTCAGGAGGGATAC
CAGAGAGTTCTGTAGGACACACGCTTATCAATAACACATACTAATTTTCTTCCCTTATAA
AGAATTTAACACGGAAAAAAGAAGAAAGAAATGAAAAACGTAGATGGCTTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGGTT
ATTAT
   
Sequence Name 14-K117775-0022-928-G02C-8409
GenBank Accession LN492696 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117775-0022-928-G02C-8409
CATTTCATTGAAGCCTCTACGTTTTTCATTTCTTTCTTCTTTTTTCCGTGTTAAATTCTT
TATAAGGGAAGAAAATTAGTATGTGTTATTGATAAGCGTGTGTCCTACAGAACTCTCTGG
TATCCCTCCTGAAACTGTGGACCGACTTGTCCCAGAACATGCCCGTAAGCAGAACCACTT
CATGGCATTACACTCGTAAGTCTCTTCCGTGTTTTCTGTACGACCTATCGGGTGTTCTCT
TCTTTTCTCTAAGCTCTCTAGATCCCAGTCTTTGGGAAAAACCAGTAGAGCTCTCTCTCT
GTAAACAGAAATCACTGGAAAAAGAAGCAATGACTTTTGTATTAAGGTGCTTGCGCATCC
CTTGTCGTGAAGTGAGGATGCGTAAAATACAACACTACTTTGTTAGATATATGATATAGA
GGAACATACTTACATTTGCAAGACTCCTGCCACAACACAATTTTCCAAAAAATTTGTGAA
CCTTAAAATGAATTGACAGTTTAGTTTATTTGTCCATGTTTACGAACTTGATTTGAACCA
TATTACTCGAATTACACCAAACCAATTTATCTTCGAGTTAATTTCAAATAAGGCATTTGT
TTTACAAAATAAGGCTTAAATATTGAAGGCCCAATAAGACCATGTCCACAAA

Last Updated on 06.12.2018 16:11