Sequences from confirmation amplicon


Confirmation sequences for line 929C12 and hit At2g15970 are shown.
Line ID 929C12
Locus At2g15970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6950972
Primers used for PCR BG23 - 8474, show primer details
   
Sequence Name 30-K101744-0022-929-C12C-8409
GenBank Accession LN499654 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K101744-0022-929-C12C-8409
ATGATCCCGACGGTGTTGGAGATACCATTGGCTTTAGTAAATGCATTTCTGAATCCACCT
GAAGCTCTAATGTGTTCTTGAAGCAAGTAACATCCAATACCTAAACATATAGCTAAACCA
ATCCAACTGTCTCTGAATGTGCCAGCAATCAAATTTGGTGCCACCACGGCGAGAAGAACC
AAAGCCACCGGTAATTCCAGGTATTCTAATAACACAAACAACAGAGATAAGCCAAATCAG
AATCAAAGTCTTGTTGCTTGTAATGGAGAGTGAATGAAAAAAAGTAGACAAACCTCTGGC
GTGTTTAGGAAAGAAGAGTTGTACAACAACAGCTACGAAAGCAATCCATTTACCAATTTC
TCCTCTGTCAAAGTTTCAGACACAAGTTAGGGTTTAGGAACATAATGATAAGTAAAGAAA
AAGTCTCTGGCTTTTGTTGTGTTATACCTGAAAATACCAAAGATCAAGGAAGGAAGACTG
AAGAAGATGTAAGGAATGAGAAGTGAGTTGAGCAT
   
Sequence Name 30-K101750-0022-929-C12C-BG23
GenBank Accession LN499655 [GenBank]
Sequencing primer gene specific primer, BG23: 5'-TATGCTCACATCACTTCTCATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K101750-0022-929-C12C-BG23
TTTGGTATTTCGGTATAACACAACAAAAGCCAGAGACTTTTTCTTTACTTATCATTATGT
TCCTAAACCCTAACTTGTGTCTGAAACTTTGACAGAGGAGAAATTGGTAAATGGATTGCT
TTCGTAGCTGTTGTTGTACAACTCTTCTTTCCTAAACACGCCAGAGGTTTGTCTACTTTT
TTTCATTCACTCTCCATTACAAGCAACAAGACTTTGATTCTGATTTGGCTTATCTCTGTT
GTTTGTGTTATTAGAATACCTGGAATTACCGGTGGCTTTGGTTCTTCTCGCCGTGGTGGC
ACCAAATTTGATTGCTGGCACATTCAGAGACAGTTGGATTGGTTTAGCTATATGTTTAGG
TATTGGATGTTACTTGCTTCAAGAACACATTAGAGCTTCAGGTGGATTCAGAAATGCATT
TACTAAAGCCAATGGTATCTCCAACACCGTCGGGATCATCTGTCTCGTCGTCTTCCCCGT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11