Sequences from confirmation amplicon


Confirmation sequences for line 929H02 and hit At5g42670 are shown.
Line ID 929H02
Locus At5g42670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17110434
Primers used for PCR 62xk - 8474, show primer details
   
Sequence Name 26-K119110-0022-929-H02C-62xk
GenBank Accession LN498151 [GenBank]
Sequencing primer gene specific primer, 62xk: 5'-TCTCCCTCTCACTTTTACCTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K119110-0022-929-H02C-62xk
CATTTACTTGAGAGATATAACAAAGTTGAATAGCGACATTTAACTTGTTGACTGATCTGG
ATTATTGGGCCGAGTCACGCAAAGATCATTCTTGTTAGTTCCCAAAGATATCTCCAAACA
TGGCTGAGAGATCTCCAATTCTCTACATTATCATTGGCCTTTGATGAAAATCTAGATCAT
TCTTGTTAGTTCCAAAGTTCATGCTATATAGTATATAGTTTTGTCTATTCAATACAGGTA
AAAAGTTGGCTCAAAGTTAAATGTGCCATTAAGATACAGAGAAAGTGCTGTAAAAGTATC
AACCACCAAAGCACAAAGGAACAAAGAAACAGAAATATCTAAAAGAACAGACATTTTCAA
AAACAGAGTTATGTTCAGTTTTCAATCTTCTCCACCTGAGGCCTTTTGCTCATTTACATT
TTCTAGTCAATATATTCCACCTGAGGACTGCCAAAAAATGTATCTTAGAAATAGTGTGGA
CGACGAATCCATCAGAAATGATAAAAGGAAACAACTATGGATAAATTTATGAATTTAACT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCG
   
Sequence Name 03-K119109-0022-929-H02C-8409
GenBank Accession LN487702 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119109-0022-929-H02C-8409
AGTTAAATTCATAAATTTATCCATAGTTGTTTCCTTTTATCATTTCTGATGGATTCGTCG
TCCACACTATTTCTAAGATACATTTTTTGGCAGTCCTCAGGTGGAATATATTGACTAGAA
AATGTAAATGAGCAAAAGGCCTCAGGTGGAGAAGATTGAAAACTGAACATAACTCTGTTT
TTGAAAATGTCTGTTCTTTTAGATATTTCTGTTTCTTTGTTCCTTTGTGCTTTGGTGGTT
GATACTTTTACAGCACTTTCTCTGTATCTTAATGGCACATTTAACTTTGAGCCAACTTTT
TACCTGTATTGAATAGACAAAACTATATACTATATAGCATGAACTTTGGAACTAACAAGA
ATGATCTAGATTTTCATCAAAGGCCAATGATAATGTAGAGAATTGGAGATCTCTCAGCCA
TGTTTGGAGATATCTTTGGGAACTAACAAGAATGATCTTTGCGTGACTCGGCCCAATAAT
CCAGATCAGTCAACAAGTTAAATGTCGCTATTCAACTTTTGTTATTATCTCTCTAAGTTA
TAATTGCCTTAGACCTAGTAACAAAAAAACAAGGTAAAATGAAGAGGGAG

Last Updated on 06.12.2018 16:11