Sequences from confirmation amplicon


Confirmation sequences for line 930G09 and hit At1g54940 are shown.
Line ID 930G09
Locus At1g54940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20483645
Primers used for PCR 45vf - 8474, show primer details
   
Sequence Name 21-K117874-0022-930-G09C-45vf
GenBank Accession LN495844 [GenBank]
Sequencing primer gene specific primer, 45vf: 5'-TGTGCCACCATACAAATATCTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117874-0022-930-G09C-45vf
CTCATCTTGACGATTTCTCCTTAAATCTTTAAACATACATGCCGATGGCTCGAGAACCTA
GATGTCAAGAGAAAAACAATTAGTACATTCTTAGCTAATACATTTTTTCCCCCGAAAACC
ACAAAATATAAATTTTGTTTTTTAAAAAAAAAAAAAATTTGTTTTCAAGGGCAAAAAAAA
AAAAAAATTTTTTAACCGAAAACCAAAAAACAAGGTTTTTTAAAACAAATTTTTTCAAGG
GAATGTGGGGAAAAATTTTTTTTAAAAAATTTAAGAATTTTAGGGCAAAATTTTAAAACA
TTTTTGGGGCCCAAAAAATTTTTTAAAAATTTAAAAGGTTTCCCCAAAAAATAAAATTAA
TTTAAAAACCAGTTTTGGGGGGAATACCCAAAATCCGGGATTTAAAAAATTTTTTCTATT
CCCAAAAAAAAAAATTTGGGGTAATCAAAAAAAAAAAATCATTTTTCAGGAGAATTTTTT
CATTTTAAAGGGGTTTTGGGCCGGGAAAATCTCTTGGGATAACCAGGTTCTATAAGGGCA
AATTTGGAAAAAAAAAAAAAGCATTCCCCAATTTTTTTCCATTCAAAAAGGGAAAATTCC
   
Sequence Name 09-K117873-0022-930-G09C-8409
GenBank Accession LN490382 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117873-0022-930-G09C-8409
CATTACATTGAATATATCCTCAAGAAACTTGATCATCTCTTCTACTATCCACAACTCTCA
GCTTCAGGCAACGACAAAGTGTTATTCAACTCCGGAATCATGGTAATGCAGCCAAATACT
GGTCTTAAATTAATTTTACTTTTTTGGTAAAACGTTCTAAATGATAAAAAATGATATGGT
CATCTATATATGTTGACGTATTTGGCCTAAAAATACTAAATATTTTAAACAAAATTCATG
CCACACATCCCTTGAACAGAATTTGTTGAAAATTGGTTTGTTTTTGGTTTGCTGATCAAA
TATTCTTTTTTTTTTTCTGCCTTAAAAAACAATTTTTTTTTTTTTTAAAAAAAAAAATTT
TTTTTTTGGGGTTTTCGGGGGAAAAAAATTTTTTACCAAAAAAGGTCCAATTTTTTTTTC
CCTAAAAACAGGTTTCCGGACCCTCCGGCTTTTTTTTTAAAAATTTTAGGGAAAAATTCT
TTAAAAATTGGTTCTACCACGGGAGAAAACCAGGGTTCCTTAAAGAAAATTTTTGGGGGG
GGGCAAAAA

Last Updated on 06.12.2018 16:11