Sequences from confirmation amplicon


Confirmation sequences for line 932F08 and hit At1g08350 are shown.
Line ID 932F08
Locus At1g08350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2634887
Primers used for PCR 59sa - 8474, show primer details
   
Sequence Name 63-K116321-0022-932-F08C-59sa
GenBank Accession LN511065 [GenBank]
Sequencing primer gene specific primer, 59sa: 5'-TGCTTCCTTTCTCTCTTCTTCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K116321-0022-932-F08C-59sa
AAGGAGAGTTAAACTCAAAAATGTAAATTTTATCACCACATCCGTTAAACTACGCAAAAC
AAGAATAGTCTCCTAAGAAAAAATTGTCACCTTCTTAGTTCATTCTTGAGATGGCGCATG
AAGAGAAAAGATATAAGTCCAATTAACAAAACAACAACAGTTATCGAGTTGAGAAATGAG
AAGAAGTGAATCTTCTGGCTGATAGGATGAAACGACGCCCGGGAGTACTTATTCATTCTT
GTCTCAGAGCGTTCTGAAGTCAGATTCCAAGAAACCGAGTATGTAAATTGAACATCAATC
TCAGTGTTCTCGGATATATCAACCATATAACTCGGATCACTAAAACTGTTGATCTCAATA
ACTTTGTCTGCATTGTACAGAACATTGAACTTTAAGTGACTGAATATATAATACTTAGTA
TGCTTCTCCCCTTGACCAAAATAATCTCCCTCTACATGGATCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 31-K116320-0022-932-F08C-8409
GenBank Accession LN500306 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K116320-0022-932-F08C-8409
GCATTACATTGATCCTGTAGAGGGAGATTATTTTGGTCAAGGGGAGAAGCATACTAAGTA
TTATATATTCAGTCACTTAAAGTTCAATGTTCTGTACAATGCAGACAAAGTTATTGAGAT
CAACAGTTTTAGTGATCCGAGTTATATGGTTGATATATCCGAGAACACTGAGATTGATGT
TCAATTTACATACTCGGTTTCTTGGAATCTGACTTCAGAACGCTCTGAGACAAGAATGAA
TAAGTACTCCCGGGCGTCGTTTCATCCTATCAGCCAGAAGATTCACTTCTTCTCATTTCT
CAACTCGATAACTGTTGTTGTTTTGTTAATTGGACTTATATCTTTTCTCTTCATGCGCCA
TCTCAAGAATGAACTAAGAAGGTGACAATTTTTTCTTAGGAGACTATTCTTGTTTTGCGT
AGTTTAACGGATGTGGTGATAAAATTTACATTTTTGAGTTTAACTTCTCCTTTATGCATT
TCAGTTACTCTATCGGAGATGAAGAAG

Last Updated on 06.12.2018 16:11