Sequences from confirmation amplicon


Confirmation sequences for line 932H05 and hit At1g52930 are shown.
Line ID 932H05
Locus At1g52930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19711475
Primers used for PCR 63hw - 8474, show primer details
   
Sequence Name 63-K112689-0022-932-H05C-63hw
GenBank Accession LN511024 [GenBank]
Sequencing primer gene specific primer, 63hw: 5'-CAACAAACCAAACCCTAACAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K112689-0022-932-H05C-63hw
ACAATACTAATACCAATAGAGTCAAGATTGTCCATTTGCATCAATATGCATATACATAAG
CTTCCATCGAGTATTCTAATCACAAGTTTCTGAGGTTTGACTTTTGAGTCAAACATTCCA
CAGTAAAGGAACAAATCTGACTACATGGAACAAAAACTAAGCCTAGAAATAAGTAAGATG
GAAACTTTACCTCGAAGAACAAGCAGGAATTAGAATTCTTAAGCTCAATGAGCTCATTCA
AAGTCGCGCCTTTGCTACTCTTTGCTTCAACTTTACTGTCTTTCTTACAGTGTGGTAGAA
GTGAAACAATGTTCAACATCAAACTCCGATACCTTCACACAAAAATCCCCAATAAACAAA
TCAATTTCAAACCCTAAAAACGAAATCAAAACCAAAAGAAAGGAAACAAATTTAGCAATT
TAAACTCAACCTGAAACTGATACGGGAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATTC
   
Sequence Name 31-K112688-0022-932-H05C-8409
GenBank Accession LN500232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112688-0022-932-H05C-8409
GTTTAATTGCTAAATTTGTTTCCTTTCTTTTGGTTTTGATTTCGTTTTTAGGGTTTGAAA
TTGATTTGTTTATTGGGGATTTTTGTGTGAAGGTATCGGAGTTTGATGTTGAACATTGTT
TCACTTCTACCACACTGTAAGAAAGACAGTAAAGTTGAAGCAAAGAGTAGCAAAGGCGCG
ACTTTGAATGAGCTCATTGAGCTTAAGAATTCTAATTCCTGCTTGTTCTTCGAGGTAAAG
TTTCCATCTTACTTATTTCTAGGCTTAGTTTTTGTTCCATGTAGTCAGATTTGTTCCTTT
ACTGTGGAATGTTTGACTCAAAAGTCAAACCTCAGAAACTTGTGATTAGAATACTCGATG
GAAGCTTATGTATATGCATATTGATGCAAATGGACAATCTTTGACTCTATTGGTATTTAG
TATTTGTTCCATGTCATCAAAATTTGATAGTTATCCCAAATTGTTAG

Last Updated on 06.12.2018 16:11