Sequences from confirmation amplicon


Confirmation sequences for line 933D03 and hit At1g17650 are shown.
Line ID 933D03
Locus At1g17650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6070517
Primers used for PCR EP57 - 8474, show primer details
   
Sequence Name 61-K103311-0022-933-D03C-8409
GenBank Accession LN510493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K103311-0022-933-D03C-8409
GAGCCATTTACATTGAATATATCCTGCCGCCGCTGCCGCTTTGATTTCCTGCGATCATCA
CTTTTGCATATCAGATTATTTTGTCTTCGCGGGTGACTTGCCTGTATTTTCTAACATGGT
TGAATCATGAATGAAATGGAGATTTATGACTATCTGGCACTTGACATTTTAGGTGACAAG
CCGCTATACGAAAAAGCTGCTCCTTTCTTAGACATCATGGGAAAGGTATCTCTTTTGCCT
GTGTTTTGCTTTTAGTTCTTTTTTCTTCTGCAAATGAACCATTTTCGTTACTAGTTTGCA
TTATATGATCCAGTCAAAATTCTATTTGGGTGAAGTTGGTAATGGAGCAGCAATGAAACT
TGTCGTCAACATGATCATGGGAAGGTATCATTTGACCATC
   
Sequence Name 54-K103312-0022-933-D03C-EP57
GenBank Accession LN508446 [GenBank]
Sequencing primer gene specific primer, EP57: 5'-GCGATAGATGGTCAAATGATACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103312-0022-933-D03C-EP57
GTTTCTTGCTGCTCCATTACCAACTTCACCCAATAGAATTTTGACTGGATCATATAATGC
AAACTAGTAACGAAAATGGTTCATTTGCAGAAGAAAAAAGAACTAAAAGCAAAACACAGG
CAAAAGAGATACCTTTCCCATGATGTCTAAGAAAGGAGCAGCTTTTTCGTATAGCGGCTT
GTCACCTAAAATGTCAAGTGCCAGATAGTCATAAATCTCCATTTCATTCATGATTCAACC
ATGTTAGAAAATACAGGCAAGTCACCCGCGAAGACAAAATAATCTGATATGCAAAAGTGA
TGATCGCAGGAAATCAAAGCGGCAGCGGCGGCNGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCGCAC

Last Updated on 06.12.2018 16:11