Genotyping and primer details


Genotyping and primer details for 933D03 - At1g17650
Line ID 933D03
Locus At1g17650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6070517 of line 933D03 Chr1:6070517
   
Insertion allele amplicon 8474 - EP57
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EP57: 5'-GCGATAGATGGTCAAATGATACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6070163
   
wt amplicon (over InsPos) 11id - EP57
wt amplicon start Chr1:6070163
wt amplicon end Chr1:6070840
wt amplicon size 678 bp
Locus specific primer EP57: 5'-GCGATAGATGGTCAAATGATACCT-3'
2nd locus specific primer 11id: 5'-TCGATACTTTCTGGTAAAAATGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11