Sequences from confirmation amplicon


Confirmation sequences for line 934D03 and hit At4g39270 are shown.
Line ID 934D03
Locus At4g39270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18277882
Primers used for PCR EU99 - 8474, show primer details
   
Sequence Name 44-K103927-0022-934-D03C-EU99
GenBank Accession LN505162 [GenBank]
Sequencing primer gene specific primer, EU99: 5'-GAACTCCGTTTTCTAGAACTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103927-0022-934-D03C-EU99
GATCGGTTTGAATCCTGAACTCCTTGGTGGCATTGAGCAACTGCTGATAGGTAAATGATG
ATCCAAGACTCCCAAAGTTTATAGAGACTCCTCCTCTTGAGGGAAGTGTTTCATCAGGAG
GTAATGGGCCAACACCGTTGTGTCTTCCTCTAGGATGATTACTTGTTGATGATCTGTTTC
TTCGACGGACACAGAAACTCACTGTTATTGGTAACACTATGAGAATGAGCATAAGCAATA
TAGACCCTCCAACTGCAGCAAGTATAACTATTTTCGTGTGGCTTAGCCAAGAAGTTTTAG
AACTCTTCGTCTCTTCATGTTGTC
   
Sequence Name 60-K103928-0022-934-D03C-8409
GenBank Accession LN510243 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103928-0022-934-D03C-8409
CCGGAATGAGCCATTTACAATTGAATATATCCTGTTCGGGAAGAGGCGATACTAGGGTTC
CTTTGAGTTACACTTTTACTGACAAGGTTCCAAGTTGTTGACCTGTCAGAAAACTATTTT
GAAGGTAAAATTCCTGACTTTGTGCCAACCAGAGCTTCATTGAGCAACAATTGTCTTCAG
GGCCCAGAGAAGCAGCGGAAGTTGTCGGATTGCACTTTGTTTTATTCAAAAAAGGGCTTA
ACTTTCAACAATTTCGGACAACATGAAGAGAAGAAGAGTTCTAAAACTTCCTGGTTAAGC
CACACGAAAATAGTTATACTTGCTGCAGTTGGAGGGTCTATATTGCTTATGCTCACTCTC
ATAGTGTTACCAATAACAGTGAGTTTCTGTGTCCGT

Last Updated on 06.12.2018 16:11