Sequences from confirmation amplicon


Confirmation sequences for line 938D07 and hit At4g32800 are shown.
Line ID 938D07
Locus At4g32800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15820210
Primers used for PCR 29lq - 8474, show primer details
   
Sequence Name 15-K114086-0022-938-D07C-29lq
GenBank Accession LN493061 [GenBank]
Sequencing primer gene specific primer, 29lq: 5'-TTACACCCCAAAAGAAAATGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114086-0022-938-D07C-29lq
TTCTTTACACAACCCAAATCTACAAAAAATCCCAATAAAAAAAAACTTCAGTCTGTTTGA
CATTTTGTCGAACACTTGGACGGCATCACAAAAAGCTCTAAACTTTCTGACTACCAATGG
CGGATTCGTCTTCCGACAAGGAGAAGAAGGAAAATAATAAGCAGCCCGTGTATCGTGGAG
TCCGTATGAGGAGCTGGGGAAAATGGGTATCGGAGATTCGCGAACCGAGGAAGAAATCGA
GAATCTGGCTCGGGACTTTTCCGACGGCGGAGATGGCTATGCGTGCTCACGACGTGGCGG
CTATGAGTATCAAAGGAACTTCAGCCATTCTCAATTTCCCTGAGCTCTCTAAACTTCTTC
CCCGACCCGTTTCGCTCAGCCCTCGTGACGTCAGAGCTGCGGCGACCAAAGCTGCTCTCA
TGGACTTCGATACGACGGCGTTTCGTTCGGATACTGAGACTAGCGAAACGACGACGTCGA
ATAAAATGTCAGAGAGTAGTGAGAGTAACGAAACGAGTCTCTCTGTTACGAGCATTGAGG
AAAGTACAGTCTCCGACGATCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCA
   
Sequence Name 39-K114085-0022-938-D07C-8409
GenBank Accession LN503430 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K114085-0022-938-D07C-8409
CGGAATGAGATCGTCGGAGACTGTACTTTCCTCAATGCTCGTAACAGAGAGACTCGTTTC
GTTACTCTCACTACTCTCTGACATTTTATTCGACGTCGTCGTTTCGCTAGTCTCAGTATC
CGAACGAAACGCCGTCGTATCGAAGTCCATGAGAGCAGCTTTGGTCGCCGCAGCTCTGAC
GTCACGAGGGCTGAGCGAAACGGGTCGGGGAAGAAGTTTAGAGAGCTCAGGGAAATTGAG
AATGGCTGAAGTTCCTTTGATACTCATAGCCGCCACGTCGTGAGCACGCATAGCCATCTC
CGCCGTCGGAAAAGTCCCGAGCCAGATTCTCGATTTCTTCCTCGGTTCGCGAATCTCCGA
TACCCATTTTCCCCAGCTCCTCATACGGACTCCACGATACACGGGCTGCTTATTATTTTC
CTTCTTCTCCTTGTCGGAAGACGAATCCGCCATTGGTAGTCAGAAAGTTTAGAGCTTTTT
GTGATGCCGTCCAAGTGTTCGACAAAATGTCAAACAGACTGAAGTTTTTTTTTATTGGGA
TTTTTTGTAGATTTGGGTTGTTGTTGAAAGAAATATTATAAGGCAAAACGAGTGAGACAT
TTTCTTTGGNGGTGTAA

Last Updated on 06.12.2018 16:11