Sequences from confirmation amplicon


Confirmation sequences for line 939H01 and hit At1g08990 are shown.
Line ID 939H01
Locus At1g08990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2889652
Primers used for PCR 46vf - 8474, show primer details
   
Sequence Name 22-K117874-0022-939-H01C-46vf
GenBank Accession LN496294 [GenBank]
Sequencing primer gene specific primer, 46vf: 5'-TGAACAAGACTTTGTTATTGCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117874-0022-939-H01C-46vf
GGTATCATATTCTTGACATGATGAAGTCTGCGTCTATAACACTAGTTTGTCGTAATCTGT
CACTTGCCACACACGTAACTTACTGTAGTTCCACTCATTGTAAGAACGCTTCTTGGAAAA
AGGACTACGAATTCTCTCCACTCGCCGTAGTTTCCAGCCAGCAAGGCTTAGGCCAATGAG
AGAGATGTTGGTTATAGAGTCATCGTGGAGGAGAATCATGTCCTTGGTTGATCCAGATTG
CCTTATGCTTTGTGCTAAGGCTATTGCTCCGCATACGTATACCTCCGATGAGTGTAGTAA
CGTCACGTAAGCGACACGTTGGGCAGTAAATGATGATAATGTAGTTTTTTCGGATGTGAG
ATTTCTGTTCTTGTCTTGTATCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 10-K117873-0022-939-H01C-8409
GenBank Accession LN490851 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117873-0022-939-H01C-8409
CCATTTACAATTGGATACAGACAAGAACAGAAATCTCACATCCGAAAAAACTACATTATC
ATCATTTACTGCCCAACGTGTCGCTTACGTGACGTTACTACACTCATCGGAGGTATACGT
ATGCGGAGCAATAGCCTTAGCACAAAGCATAAGGCAATCTGGATCAACCAAGGACATGAT
TCTCCTCCACGATGACTCTATAACCAACATCTCTCTCATTGGCCTAAGCCTTGCTGGCTG
GAAACTACGGCGAGTGGAGAGAATTCGTAGTCCTTTTTCCAAGAAGCGTTCTTACAATGA
GTGGAACTACAGTAAGTTACGTGTGTGGCAAGTGACAGATTACGACAAACTAGTGTTTAT
AGACGCAGACTTCATCATCGTCAAGAATATTGATTACCTTTTCTCCTATCCTCAACTTTC
TGCCGCTGGCAATAACAAGTCCTTGTTCA

Last Updated on 06.12.2018 16:11