Sequences from confirmation amplicon


Confirmation sequences for line 942E05 and hit At1g52660 are shown.
Line ID 942E05
Locus At1g52660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19614266
Primers used for PCR 35oh - 8474, show primer details
   
Sequence Name 52-K114805-0022-942-E05C-35oh
GenBank Accession LN507903 [GenBank]
Sequencing primer gene specific primer, 35oh: 5'-TTGACCTGAGAAATTAGGAGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K114805-0022-942-E05C-35oh
GAGGCCATGGCTCGGCCAATGGTGACCAAAGCTAGAGGAAGACCGTCGCACCTGGCTGCA
ACCTCTTGAGCCACTTTGGCTATGTCTGGGTGGCTCTTTATAGTATCTTCTCCAACATTC
TTCTTAAACAAGTCCCAAGCTCGTTCCCATGCCAATTTCTCTACTTTGATCTTTGTCTGT
GCACTCATCTCCTTACAAACTTCTTCGGAACATGTCGTGAACACTATCTTCGACCGGTTC
TGCGCATCCNGTGGCGGAACTCCAGCTTTCACTGTAAAAGAAAAGCAGATCATTTATATC
ACAAGTAATATAAACACTATTGCTTGCACCGTAAGTAATCTTTACAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 28-K114804-0022-942-E05C-8409
GenBank Accession LN498967 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114804-0022-942-E05C-8409
ATTACTTACGGGTGCAAGCAATAGTGTTTATATTACTTGTGATATAAATGATCTGCTTTT
CTTTTACAGTGAAAGCTGGAGTTCCGCCACCGGATGCGCAGAACCGGTCGAAGATAGTGT
TCACGACATGTTCCGAAGAAGTTTGTAAGGAGATGAGTGCACAGACAAAGATCAAAGTAG
AGAAATTGGCATGGGAACGAGCTTGGGACTTGTTTAAGAAGAATGTTGGAGAAGATACTA
TAAAGAGCCACCCAGACATAGCCAAAGTGGCTCAAGAGGTTGCAGCCAGGTGCGACGGTC
TTCCTCTAGCTTTGGTCACCATTGGCCGAGCCATGGCCTCCAAGAAAACCCCTCAAGAAT
GGCGTGACGCCTTGTACATCTNGAGTAACTCTCCTCCTAAT

Last Updated on 06.12.2018 16:11