Sequences from confirmation amplicon


Confirmation sequences for line 943B09 and hit At2g34820 are shown.
Line ID 943B09
Locus At2g34820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14690400
Primers used for PCR 27cv - 8474, show primer details
   
Sequence Name 18-K109134-0022-943-B09C-27cv
GenBank Accession LN494341 [GenBank]
Sequencing primer gene specific primer, 27cv: 5'-TCCCTAACCCTAAAAACGAAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K109134-0022-943-B09C-27cv
GACTCGATTTCTTTCTCCCCGCCAAACGCCAGAGACTTGTTAACTCCAGCTACAATTGTA
ACACTCAAAACCATTTCCAGAGCCGTAACCCGAATTTCTTCGACCCTTTCGGCGACACTG
ATTTCGTTCCAGAATCTTGTACCTTCCAGGAGTTTCGAGTTCCGGATTTCTCTTTAGCTT
TCAAGGTAGGCCGGGGAGATCAAGATGACTCAAAGAAACCGACGCTTTCATCTCAGAGCA
TCGCGGCTAGAGGGAGGAGAAGAAGAATTGCAGAGAAGACTCACGAGCTCGGAAAACTCA
TCCCCGGTGGCAATAAACTTAACACCGCCGAGATGTTCCAAGCCGCCGCTAAGTATGTCA
AGTTTTTGCAGAGTCAAGTTGGGATTCTCCAACTGATGCAGACCACAAAGAAGGTAATAA
CCAACCCCAAATAAGAACTTTATCATCCAATTGAAACTCTAATCGTGTTTTCTCACAAGC
TTCTTAATTTGTTTACGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 02-K109133-0022-943-B09C-8409
GenBank Accession LN487069 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K109133-0022-943-B09C-8409
CATTTACAATTGAATATATCCTGCGTAAACAAATTAAGAAGCTTGTGAGAACACTCGATT
AGAGTTTCAATTGGATGATAAAGTTCTTATTTGGGGTTGGTTATTACCTTCTTTGTGGTC
TGCATCAGTTGGAGAATCCCAACTTGACTCTGCAAAAACTTGACATACTTAGCGGCGGCT
TGGAACATCTCGGCGGTGTTAAGTTTATTGCCACCGGGGATGAGTTTTCCGAGCTCGTGA
GTCTTCTCTGCAATTCTTCTTCTCCTCCCTCTAGCCGCGATGCTCTGAGATGAAAGCGTC
GGTTTCTTTGAGTCATCTTGATCTCCCCGGCCTACCTTGAAAGCTAAAGAGAAATCCGGA
ACTCGAAACTCCTGGAAGGTACAAGATTCTGGAACGAAATCAGTGTCGCCGAAAGGGTCG
AAGAAATTCGGGTTACGGCTCTGGAAATGGTTTTGAGTGTTACAATTGTAGCTGGAGTTA
ACAAGTCTCTGGCGTTTTGGCGTGGGGAGAAAGAAATCGAGGTCGTGTGTTTCGTTGAAG
ATTTCGTTTTTA

Last Updated on 06.12.2018 16:11