Sequences from confirmation amplicon


Confirmation sequences for line 944B10 and hit At3g48400 are shown.
Line ID 944B10
Locus At3g48400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17924356
Primers used for PCR 39qp - 8474, show primer details
   
Sequence Name 73-K115714-0022-944-B10C-39qp
GenBank Accession LN512957 [GenBank]
Sequencing primer gene specific primer, 39qp: 5'-GATCTGACGATAGAGGAAGGACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K115714-0022-944-B10C-39qp
GATGATACCATTCTTGTTGAGCATCAAATTATGTTTTTCATGGCTGAAATGTTTTATCGT
GTTGTCATCAACCACCTCGAATGGCGCGGTATCTTGCTCACTATCTTCAGGAGTCCCTTC
GAGTTCTATTCCGTCCCATACATCATCTTTCATTGCACATTGTGGATGAACAACATAGTT
GGGACAAAGAGAGCAAGAATAACCTCCGTAGTATCCATTAACACTTTGACGACATACTCC
ACATTCTGAATACTGAAGACCAAGATGATGCGTGAGAGAGATGCGATGATCATGACGATT
GATGTTAATGACGCGAGGTAAATCGATGCATTTCCGATGGATTAAGTAACCACATCCAAG
ACAAAAGTAAGGACATCCATCACCTTTCATTCCACAAGCATTGCAAATAAACTTAACTTG
TCTAGGCAAGAGAGTTAGCGTATGCTCGTGAGTCTTAAGATGTTCTATAGCAAGCGGTGG
AGGATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAATATG
TAGATGTCCGCA
   
Sequence Name 33-K115713-0022-944-B10C-8409
GenBank Accession LN501126 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115713-0022-944-B10C-8409
CCATTTACAATTGGTAATCCTCCACCGCTTGCTATAGAACATCTTAAGACTCACGAGCAT
ACGCTAACTCTCTTGCCTAGACAAGTTAAGTTTATTTGCAATGCTTGTGGAATGAAAGGT
GATGGATGTCCTTACTTTTGTCTTGGATGTGGTTACTTAATCCATCGGAAATGCATCGAT
TTACCTCGCGTCATTAACATCAATCGTCATGATCATCGCATCTCTCTCACGCATCATCTT
GGTCTTCAGTATTCAGAATGTGGAGTATGTCGTCAAAGTGTTAATGGATACTACGGAGGT
TATTCTTGCTCTCTTTGTCCCAACTATGTTGTTCATCCACAATGTGCAATGAAAGATGAT
GTATGGGACGGAATAGAACTCGAAGGGACTCCTGAAGATAGTGAGCAAGATACCGCGCCA
TTCGAGGTGGTTGATGACAACACGATAAAACATTTCAGCCATGAAAAACATAATTTGATG
CTCAACAAGAATGGTATCATCACTCAACATGATGAAATCACACGTTGCCNAGCATGTGTC
CTTCCTCTTCGGTCAGAT

Last Updated on 06.12.2018 16:11