Sequences from confirmation amplicon


Confirmation sequences for line 945C09 and hit At5g24160 are shown.
Line ID 945C09
Locus At5g24160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8185422
Primers used for PCR 79xm - 8474, show primer details
   
Sequence Name 61-K119407-0022-945-C09C-79xm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 79xm: 5'-ACGTTTGTTTATGGACGTTAGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K119407-0022-945-C09C-79xm
TACCAACATGAAGAAGAAGCGACGGATTTGGCTGATACGGTGGCTGAGGATCAAAAAGAC
GGTGCTGCTGACGTCATTATCGTCGGGGCTGGTGTAGGTGGTTCGGCTCTCGCATATGCT
CTTGCTAAGGTATGTAAAGTGGCGTCATAGCTTTGTGTCCAAATATTAAATCTGCATCAC
ACTTTTATTAACTACTTTCTTGTACTAAAATCTCCGTATTAATAATAGGATAAAACCTAT
ACACATTATGTATGTTTTTTTTTTGGCAAAATAAATATTGCAATATTTTGTTTTTGCATG
ACACAATCAAAATCGGCTCTAAAAATTGAAAAACCCCTAAGGATATAAAAACAAATTAAA
GCGCAATAATTAGGAATCTTTAAAAGGAAAACTAATGAAAATTGATTTAGGAAATGTGTA
ACTCTAAAACTTTTTTCGTCCACGTTATAATACCTCTAGCACTAAATTACAAGTCTACAA
AAACATTTTAGTCAAAAAGGGACCAAATGAATATAGGAAAAAATACCTTAAGGCAAAGTC
GGCTTTGAATACAAATATTATACACAATCTTTTTCCTATGCTAATATCCAACTATAGAAT
GGGCGTCAAGTACATGTGATCGAAAGGGACATGAAAAAACCAAAAAGAATGATGGGTGAG
TTTATGCAACCTGGCGAACAACTCAGGCTTTCTAAACTTGGCCTTCAAGGTCTGTGTCCG
CTTTACTTTGATTTTACATTTATGTAAACCAATCAAGAAATGTATATGGCGTCTAAGTTC
CATGATGTCATGCGTTTATAATTT
   
Sequence Name 21-K119406-0022-945-C09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K119406-0022-945-C09C-8409
GCCATTTACATCGATTACTGTATATCAGACGTATTCATGATTTTCCATTGAATATAATAA
ATCAATGACTATATATACTAAATCAATGACTATAAATATTTGTGAACAAAGAAGTATCCT
TTTGACGTACTTGGAAAGAGAAAAAGCCTTTCGACGCAGTTGTTGGACGAATCGGCCATT
GTGAAAAGATCGAGCAGAAGGTTCATAAGAAAAATTGTTGTTATCCACTGGAAAAGGTGC
GTCTGCTTCTTTTCCATCTTTATAAACTGCCAAACCCGTGGCTTTCTGTGCATCTATGTC
TTCCAAGCAATCTATATAAATTATAAACGCATGACATCATGGAACTAAGACGCAATATAC
ATTTCTTGATTGGTTTACATAAATGTAAAATCAAAGTAAAGCGGACACAGACCTTGAAGG
CCAAGTTTAGAAAGCATGAGTCGTCCGCCAGGTTGCATAAACTCACCCATCATTCTTTCT
GGTTCTCTCATGTCCCTCTCGATCACATGTACTCGACGCCCATCCTATAGTCGGATATTA
GCATAGGAAAATGATTGTGTATAATATTTGTATTCAAAGCCGACTCTGCCTTAAGGTATT
TTTTCCTATATTCATTCGGTCCCTTTTTGACTAAAATGTTCTCGTAGACTTGTAATCTAG
TGCTAGAGGTATTATAACGTGGACGAAAAAAGTTCTAGAGTTACACATCTCCTAACTCAA
TTCTCATTAGTTTTCCTTTTAAAGATTCCTAATTATTGCGCTTTAATTTGTTCTTATATC
CTTAGGCGTTTTTCAATTCTTAGAGCCGATTTTGATTGTGTCATGCAAAAACAAAATATT
GCAATATCTATCTTGC

Last Updated on 06.12.2018 16:11