Sequences from confirmation amplicon


Confirmation sequences for line 947G11 and hit At5g38650 are shown.
Line ID 947G11
Locus At5g38650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15472352
Primers used for PCR 32lu - 8474, show primer details
   
Sequence Name 17-K114086-0022-947-G11C-32lu
GenBank Accession LN493938 [GenBank]
Sequencing primer gene specific primer, 32lu: 5'-TAGCACTACACGATTATGATTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114086-0022-947-G11C-32lu
ATACACATTTGTACTAACATATTTCAAATTCAGAACAGCAATTGACCTAATCAAGATAGA
TTACTAGCATTAGGAAACCATATTTCAATCTTAACAGTATTCTATAAAGCCTAAGGAAGA
TTCTCATACATTCTCATACCTAATAATCAGTGTAATTCAGAATTTCATATGCTACAGATT
TAAAGATCCATACCGAGAAAGGATTTGTCTATCAAGATCCATCTTCAAAGGAAGAGCAGC
TCCATAAGTATGCGTGATCACTCTCCTCTTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGATTATT
   
Sequence Name 41-K114085-0022-947-G11C-8409
GenBank Accession LN504159 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114085-0022-947-G11C-8409
TTGAAGAGGAGAGTGATCACGCATACTTATGGAGCTGCTCTTCCTTTGAAGATGGATCTT
GATAGACAAATCCTTTCTCGGTATGGATCTTTAAATCTGTAGCATATGAAATTCTGAATT
ACACTGATTATTAGGTATGAGAATGTATGAGAATCTTCCTTAGGCTTTATAGAATACTGT
TAAGATTGAAATATGGTTTCCTAATGCTAGTAATCTATCTTGATTAGGTCAATTGCTGTT
CTGAATTTTGAAATATTGTTAGTACAAATGTGTATATGAATCATCATAATGATTATATCG
AAAATTCGGAATCATAATCTGNTAGTGCT

Last Updated on 06.12.2018 16:11