Sequences from confirmation amplicon


Confirmation sequences for line 950D08 and hit At5g22610 are shown.
Line ID 950D08
Locus At5g22610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7516790
Primers used for PCR 60tv - 8474, show primer details
   
Sequence Name 22-K117167-0022-950-D08C-60tv
GenBank Accession LN496284 [GenBank]
Sequencing primer gene specific primer, 60tv: 5'-ATGACAACTAACATTGAGGCACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117167-0022-950-D08C-60tv
CAAGGAGTAACCCGCTCATCTTGGAAGATGGTCCATCCCCATCACAAGACCTGCCTGAAG
AATCTTTTCCTAAATCCCGGCAAAATTACGGTAATGTATAGCAATTTTTTGCATGAAAAA
TAGTTTGGAATTCAACATCATCATCTGACCCTGAGAGCTTTGTCCAAAGTATATATAAAT
ACAAAGACATGATAACAGAATTCAACAAAATCAAAATCCAAGTCCAATCTCTAGGAGTGC
TAGTCTCGGTCATAGCACCACCTAAAAGTCTTTCTAACAGTCACTCAAATGCACAACAAG
TTTCTTGAGAACTACCGAGTTCTCTACAAAGTACCTTGCCACTTTAACTCACAATGGCAC
CATAATAGGATTCAGCAACATCAAAATAGTCTAAGAAAACACGGCCGGTTATTTTGTTTT
TGCGAAAACAAAGAGCATGGCCGTTTAGCGCATTAAAGACTTCGATCTGATCCATGCATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 11-K117166-0022-950-D08C-8409
GenBank Accession LN491297 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117166-0022-950-D08C-8409
TATGCATGGATCAGATCGAAGTCTTTAATGCGCTAAACGGCCATGCTCTTTGTTTTCGCA
AAAACAAAATAACCGGCCGTGTTTTCTTAGACTATTTTGATGTTGCTGAATCCTATTATG
GTGCCATTGTGAGTTAAAGTGGCAAGGTACTTTGTAGAGAACTCGGTAGTTCTCAAGAAA
CTTGTTGTGCATTTGAGTGACTGTTAGAAAGACTTTTAGGTGGTGCTATGACCGAGACTA
GCACTCCTAGAGATTGGACTTGGATTTTGATTTTGTTGAATTCTGTTATCATGTCTTTGT
ATTTATATATACTTTGGACAAAGCTCTCAGGGTCAGATGATGATGTTGAATTCCAAACTA
TTTTTCATGCAAAAAATTGCTATACATTACCGTAATTTTGCCGGGATTTAGGAAAAGATT
CTTCAGGCAGGTCTTGTGATGGGGATGGACCATCTTCCAAGATGAGCGGTGTTACTCCTT
TGTAGGATTTTCCACGTATGGCTCCAATAAGTTTCTCGTCTGTGCCTCAATGTAGTTTGT
CA

Last Updated on 06.12.2018 16:11