Sequences from confirmation amplicon


Confirmation sequences for line 950F08 and hit At4g27690 are shown.
Line ID 950F08
Locus At4g27690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13825308
Primers used for PCR 91ra - 8474, show primer details
   
Sequence Name 38-K116491-0022-950-F08C-91ra
GenBank Accession LN503118 [GenBank]
Sequencing primer gene specific primer, 91ra: 5'-TGTCTCGACATGAGTATTAGCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K116491-0022-950-F08C-91ra
GATCCATATTCTTCATCTTGATTCTCACAAGAAGAAAATATATTTTCCCAAGGATGACAT
CTTTTAGGTGATACCTGCAACCAGAGGCATAGAGTATATCTGTCTTATTTCCCAATCAAT
CAGTCAAATCCTTAAATCATGAAACGGATTTTGTCTTACTTGCTTTTATTGTACTCAAAC
TCGATGTGCAGGCAATCCTCAATTCCAACTTCCATCTGTACAAAAAGTAAGATTCAACAT
TGTGCCTCAAATGATCCTACTGATTTAAGCCTTAATCTTGTATGAGCTTAAGAACGCTCT
GAGTAGTAAATAAGGAAGCCTATTGACATCTCTTAAAGTAAGTAGGGAAAAAGGAACTCA
ACATTTGAATGCAAGCTTCAAGAGAAAGAGCACTCAATATCATTACTCATCAGTGAATCT
AGCAAGAGCCTATAAATTGCTTGAGTTTTAGGTCTTTTAGTTCCTATCTCTTTTTCTTAT
ATAGTTTCAAACTTAGATCCTATCACTATTAGAACTCACCTTGATGCTGTTATTAATATC
AGGAAGTGGAGCGTAGTTCCGTACCTATTTCAACAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 06-K116490-0022-950-F08C-8409
GenBank Accession LN488973 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116490-0022-950-F08C-8409
TTGTTGAATAGGTACGGAACTACGCTCCACTTCCTGATATTAATAACAGCATCAAGGTGA
GTTCTAATAGTGATAGGATCTAAGTTTGAAACTATATAAGAAAAAGAGATAGGAACTAAA
AGACCTAAAACTCAAGCAATTTATAGGCTCTTGCTAGATTCACTGATGAGTAATGATATT
GAGTGCTCTTTCTCTTGAAGCTTGCATTCAAATGTTGAGTTCCTTTTTCCCTACTTACTT
TAAGAGATGTCAATAGGCTTCCTTATTTACTACTCAGAGCGTTCTTAAGCTCATACAAGA
TTAAGGCTTAAATCAGTAGGATCATTTGAGGCACAATGTTGAATCTTACTTTTTGTACAG
ATGGAAGTTGGAATTGAGGATTGCCTGCACATCGAGTTTGAGTACAATAAAAGCAAGTAA
GACAAAATCCGTTTCATGATTTAAGGATTTGACTGATTGATTGGGAAATAAGACAGATAT
ACTCTATGCCTCTGGTTGCAGGTATCACCTAAAAGATGTCATCCTTGGGAAAATATATTT
TCTTCTTGTGAGAATCAAGATGAAGAATATGGATCTTGAGATCAGACGGCGGGAATCAAC
AGGTGCAGGTGCTAATACTCTGTTCGAGACA

Last Updated on 06.12.2018 16:11