Sequences from confirmation amplicon


Confirmation sequences for line 950H06 and hit At3g19420 are shown.
Line ID 950H06
Locus At3g19420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6733400
Primers used for PCR 23mv - 8474, show primer details
   
Sequence Name 48-K114393-0022-950-H06C-23mv
GenBank Accession LN506574 [GenBank]
Sequencing primer gene specific primer, 23mv: 5'-GTAGAAGTCCATACACTCTTCGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K114393-0022-950-H06C-23mv
GATTGGTTCCAGTGACAGATTGTTTCATACAACGTTAAAAGGAATTTCTTACCTTCAGAT
ACAACAGAAGACTGCATATCATAAGCCCTGTCCTTGCCATGCCAGCCTTACAGTGAACAA
CCACAACGTTCTCAATGTCCTCCTTTAACCATGAATATGCACTTTGGCAGAATGAGGTGA
CCAAATGGATTGGCGGGCAATTATGATCATCAAATGGGAAACTGGCCACCTGGATGCAAG
ATGATCAGCACAAAATAAGATTTATAGGGACATTAAAACGGACAGAAGACTGAGGGTAAA
TAGTAAACAACTGTGCCCCCATTATTTGAACATAATAGTTATCAACCTAGAANAAGAAAT
GATGCCGTACAGAAGAAGTGGCTACATTATCTGCAACAGAGAATTAAGCCACTTCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATATCCG
   
Sequence Name 24-K114392-0022-950-H06C-8409
GenBank Accession LN497093 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114392-0022-950-H06C-8409
ATATCCTGAAGTGGCTTAATTCTCTGTTGCAGATAATGTAGCCACTTCTTCTGTACGGCA
TCATTTCTTTTTCTAGGTTGATAACTATTATGTTCAAATAATGGGGGCACAGTTGTTTAC
TATTTACCCTCAGTCTTCTGTCCGTTTTAATGTCCCTATAAATCTTATTTTGTGCTGATC
ATCTTGCATCCAGGTGGCCAGTTTCCCATTTGATGATCATAATTGCCCGCCAATCCATTT
GGTCACCTCATTCTGCCAAAGTGCATATTCATGGTTAAAGGAGGACATTGAGAACGTTGT
GGTTGTTCACTGTAAGGCTGGCATGGCAAGGACAGGGCTTATGATATGCAGTCTTCTGTT
GTATCTGAAGGTAAGAAATTCCTTTTAACGTTGTATGAAACAATCTGTCACTGGGAACCA
ATCATGTTTCATTTTCTTTTATAGTTCTTTCCCACTGCCGAAGAGTGTATGAACTTCTA

Last Updated on 06.12.2018 16:11