Sequences from confirmation amplicon


Confirmation sequences for line 952E04 and hit At2g43160 are shown.
Line ID 952E04
Locus At2g43160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17950396
Primers used for PCR 28vd - 8474, show primer details
   
Sequence Name 23-K117874-0022-952-E04C-28vd
GenBank Accession LN496737 [GenBank]
Sequencing primer gene specific primer, 28vd: 5'-TTGCTTGTCATTGTATCTTTCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117874-0022-952-E04C-28vd
CTCGTTTGATTTCTTGTTGCTAGTGGCTTACATTGTTATTATGCTTCTCGGTAGTCTATT
AATTGGTAGTACTGGCACTTTAACTGTATTAGCTTCTGAATTTTGAAAGTTTTTATCTCG
AGAAAAAAAAATGACATGTCTTTTGGTTCATTCAAAATATGCTGAACATATCAACCTACA
TTATTTTACAACAGGTATCGCAGCTCAGCACCAGGTGGGATGTATAAGCCTTCAGGCGGA
TATGGGGATAAATATGATTACGGAAGCCGGGATGAAGAGCGAAGTAGTTATGGAAGAGAA
AGAGAATATGGTTACATCTCTGGATCATCGTCCGGGAAATACTCTTTACATTACCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCG
   
Sequence Name 11-K117873-0022-952-E04C-8409
GenBank Accession LN491309 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117873-0022-952-E04C-8409
ATTGAATATATCCTGGTAATGTAAAGAGTATTTCCCGGACGATGATCCAGAGATGTAACC
ATATTCTCTTTCTCTTCCATAACTACTTCGCTCTTCATCCCGGCTTCCGTAATCATATTT
ATCCCCATATCCGCCTGAAGGCTTATACATCCCACCTGGTGCTGAGCTGCGATACCTGTT
GTAAAATAATGTAGGTTGATATGTTCAGCATATTTTGAATGAACCAAAAGACATGTCATT
TTTTTTTCTCGAGATAAAAACTTTCAAAATTCAGAAGCTAATACAGTTAAAGTGCCAGTA
CTACCAATTAATAGACTACCGAGAAGCATAATAACAATTGTAAGCCACTAGCAACAAGAA
ATCAAACGAGTGTCCACCGAGACTCACACAGAGAGAAAACTATCCACCAGTTCCAGAAAG
ATACATGAACAAGCA

Last Updated on 06.12.2018 16:11