Sequences from confirmation amplicon


Confirmation sequences for line 953D02 and hit At3g49030 are shown.
Line ID 953D02
Locus At3g49030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18174701
Primers used for PCR 48lx - 8474, show primer details
   
Sequence Name 47-K114144-0022-953-D02C-48lx
GenBank Accession LN506265 [GenBank]
Sequencing primer gene specific primer, 48lx: 5'-CAAAAGAAAGAGAGTGGTGGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114144-0022-953-D02C-48lx
ATTGCAATCCTCAAGCTNCACCGGGGTAGCAAGTTTATTAGCCAACTCTGTTTTTCTTTC
TTTCCAGAACTCCACTTTAAGGTACTTTTTTTATTTTGTATTACATAAATTGCCTTGTTC
TCAGCTATCTTGTATCGAAAAAGGTCTAGATGGACAGAATTGGAATCCACCAAAATGTGT
ACCGGAATGTTTGTTGTTCCATCTTGAGAAATTCTTGTGGACAGGATACGAATGGCAACG
AGGAGATGAGAAAGAAGTGGCCACATACATACTAGAAAACGCAAGACTGTTGAAGAAAGC
AACTTTCTCCACAAAACGCATTGATCTGGAAAATCTCGAAAAGAGACGTGAGAAAAAGAA
AGAGTAATGACTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCG
   
Sequence Name 20-K114143-0022-953-D02C-8409
GenBank Accession LN495302 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114143-0022-953-D02C-8409
CATTACATTGAAGTCATTACTCTTTCTTTTTCTCACGTCTCTTTTCGAGATTTTCCAGAT
CAATGCGTTTTGTGGAGAAAGTTGCTTTCTTCAACAGTCTTGCGTTTTCTAGTATGTATG
TGGCCACTTCTTTCTCATCTCCTCGTTGCCATTCGTATCCTGTCCACAAGAATTTCTCAA
GATGGAACAACAAACATTCCGGTACACATTTTGGTGGATTCCAATTCTGTCCATCTAGAC
CTTTTTCGATACAAGATAGCTGAGAACAAGGCAATTTATGTAATACAAAATAAAAAAAGT
ACCTTAAAGTGGAGTTCTGGAAAGAAAGAAAAACAGAGTTGGCTAATAAACTTGCTTACC
CCGGTGAGCTTGAGGATTTGCAATTTAGGAGAACTCTCAAGCATTCTCGAAAGCAGATTC
CACCACTCTCTTTCTTTT

Last Updated on 06.12.2018 16:11