Sequences from confirmation amplicon


Confirmation sequences for line 953E06 and hit At5g02960 are shown.
Line ID 953E06
Locus At5g02960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:693454
Primers used for PCR 72lx - 8474, show primer details
   
Sequence Name 48-K114144-0022-953-E06C-72lx
GenBank Accession LN506567 [GenBank]
Sequencing primer gene specific primer, 72lx: 5'-TCTCTTGGGTTTTGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K114144-0022-953-E06C-72lx
GATGAATTTCTTATTGATTTTCTGAGTTTTAACTATGGTTTATCGAGTGAAGTTGTTCTC
AAATGTTATTTTGTGATGATTTGTTAACAGTAAGACACGTGGTATGGGAGCTGGGCGTAA
GCTGAAGAGACTCCGAATTAACCAGAGGTGGGCTGACAAGCAGTACAAGAAGTCTCACTT
GGGAAATGAGTGGAAGAAGCCTTTTGCTGGTTCTTCTCACGCTAAAGGAATTGTTCTTGA
GAAAATGTAAGTCTGATATAACATTCAAGTTTACTTTTTCTTATAATTGATTTGTTGAGT
AGAGACATTGGTCGTTGATGAGACTCTTGTGTTTGATGATGCAGTGGTATTGAGGCTAAG
CAGCCTAACTCTGCTATCCGTAAGTGTGCTAGAGTTCAGCTTATCAAGAATGGAAAGAAG
ATTGCTGCCTTTGTCCCCAATGATGGTTGTTTGAACTACATCGAGGAAAACGTAAGTCAC
ATGAAATGACTATTCAATCATCACACTATGGATTAGTTTCATTCATGAAATGACTATTCA
ATCATCACACTATGGATCAGCAATGAATCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 21-K114143-0022-953-E06C-8409
GenBank Accession LN495755 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K114143-0022-953-E06C-8409
ATTGAATATATCCTGATTCATTGCTGATCCATAGTGTGATGATTGAATAGTCATTTCATG
AATGAAACTAATCCATAGTGTGATGATTGAATAGTCATTTCATGTGACTTACGTTTTCCT
CGATGTAGTTCAAACAACCATCATTGGGGACAAAGGCAGCAATCTTCTTTCCATTCTTGA
TAAGCTGAACTCTAGCACACTTACGGATAGCAGAGTTAGGCTGCTTAGCCTCAATACCAC
TGCATCATCAAACACAAGAGTCTCATCAACGACCAATGTCTCTACTCAACAAATCAATTA
TAAGAAAAAGTAAACTTGAATGTTATATCAGACTTACATTTTCTCAAGAACAATTCCTTT
AGCGTGAGAAGAACCAGCAAAAGGCTTCTTCCACTCATTTCCCAAGTGAGACTTCTTGTA
CTGCTTGTCAGCCCACCTCTGGTTAATTCGGAGTCTCTTCAGCTTACGCCCAGCTCCCAT
ACCACGTGTCTTACTGTTAACAAATCATCACAAAATAACATTTGAGAACAACTTCACTCG
ATAAACCATAGTTAAAACTCAGAAAATCAATAAGAAATTCATCTAAATAATAACAAGACG
ATAGAAGATTTATCTCTAATACATCAGCAAAAGTGAGCTTCAAC

Last Updated on 06.12.2018 16:11