Sequences from confirmation amplicon


Confirmation sequences for line 956H09 and hit At1g55860 are shown.
Line ID 956H09
Locus At1g55860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20890504
Primers used for PCR 69tv - 8474, show primer details
   
Sequence Name 62-K117029-0022-956-H09C-69tv
GenBank Accession LN510839 [GenBank]
Sequencing primer gene specific primer, 69tv: 5'-ACACACACAATCTGGAAGAAACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117029-0022-956-H09C-69tv
ACGTTAATTTTCCATAGGAACAGGTGCAGCACTAGTAGGCACATCAGCTGACTTAGAGGT
GGTGGCCTCCATCCCAGATCCAATAATCAACATGGAATTGAGGATTTCAATGAACATATC
AACTCCATAAGTGCGCAACGAAGATTGATGTCTCAAGAATTCCTTCAAGCCCACTTGACA
AAGAACCAGGTGTATCACCTGTAAGAGCCCTCAGATAAGAGGGAGAAGTAAATATTTTCA
CAAAACACCTCAAGGCATTTCGGTCCTTCACAGCTTGAAGACCGCTATTGTTGAGACACA
GAGCATCCAGACACTGCGGTATGCATGTTATGGCTTCTGCAGAGCAGATGACCTCATCAG
ATATAGCATCAAGAAAGGTAGAAGTTAAACCAGCTGAATCTAAAGCATTGAAACAAGTGG
GATCTTTATGCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
T
   
Sequence Name 30-K117028-0022-956-H09C-8409
GenBank Accession LN499883 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117028-0022-956-H09C-8409
TTACAGCATAAAGATCCCACTTGTTTCAATGCTTTAGATTCAGCTGGTTTAACTTCTACC
TTTCTTGATGCTATATCTGATGAGGTCATCTGCTCTGCAGAAGCCATAACATGCATACCG
CAGTGTCTGGATGCTCTGTGTCTCAACAATAGCGGTCTTCAAGCTGTGAAGGACCGAAAT
GCCTTGAGGTGTTTTGTGAAAATATTTACTTCTCCCTCTTATCTGAGGGCTCTTACAGGT
GATACACCTGGTTCTTTGTCAAGTGGGCTTGATGAACTCTTGAGACATCAATCTTCGTTG
CGCACTTATGGAGTTGATATGTTCATTGAAATCCTCAATTCCATGTTGATTATTGGATCT
GGGATGGAGGCCACCACCTCTAAGTCAGCTGATGTGCCTACTAGTGCTGCACCTGTTCCT
ATGGAAATTGACGTTGATGAGAAGAGCTTGGCAGTTTCGGATGAGGCGGAACCATCTTCT
GACACTTCTCCAGCAAACATTGAGCTGTTTCTTCCAATGGGGGTGTG

Last Updated on 06.12.2018 16:11