Sequences from confirmation amplicon


Confirmation sequences for line 959B07 and hit At4g35470 are shown.
Line ID 959B07
Locus At4g35470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16848433
Primers used for PCR 42et - 8474, show primer details
   
Sequence Name 24-K110827-0022-959-B07C-42et
GenBank Accession LN497042 [GenBank]
Sequencing primer gene specific primer, 42et: 5'-CGTTGAGACAAATGATATCGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K110827-0022-959-B07C-42et
AACTCCGTGCAGATTATAACAAACTAAAGGCTCTTCCAGAAGCTATTGGGAAGATTACTA
CATTAGAGATCTTGTCTGTGCGTTACAATAACATCAGGCAGTTACCTACGACGATGTCTT
CTTTAGCTAGCCTCAAAGAGCTGGATGTGAGTTTCAACGAGCTCGAGTCAGTGCCAGAGA
GTTTGTGTTTTGCCACTACACTTGTGAAACTGAATATTGGAAACAATTTCGCTGACATGG
TATCGCTCCCGAGATCAATAGGCAACCTTGAGATGCTTGAAGAGCTTGATATAAGCAATA
ATCAGATCCGTGTTCTACCTGACTCCTTCAAAATGCTTACAAAGTTGCGTGTGTTTCGTG
CTCAGGAGAATCCACTACACATTCCTCCTCGTGATATAGCTGAAAAGGGCCCTCAGGTTC
TGTTCTCTCTCTCTGTGTCTCTCTATGTCATCATGTGTCTAGATTCTAAATGGTACTCAC
TCACGGATGAGTTCATCATACTTACAGGCCGTTGTTCAATACATGAATGATCTTGTGGAG
ACGAGAAACGCGAAATCTCTAATGGTTAAGCCAAAGAAGAGCTGGGTCCAGATGTGCTTC
TTCTCGAAATCCAACAAGAGAAAACAGAGCAGCATGACTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 08-K110826-0022-959-B07C-8409
GenBank Accession LN489783 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K110826-0022-959-B07C-8409
GCTGCTCTGTTTTCTCTTGTTGGATTTCGAGAAGAAGCACATCTGGACCCAGCTCTTCTT
TGGCTTAACCATTAGAGATTTCGCGTTTCTCGTCTCCACAAGATCATTCATGTATTGAAC
AACGGCCTGTAAGTATGATGAACTCATCCGTGAGTGAGTACCATTTAGAATCTAGACACA
TGATGACATAGAGAGACACAGAGAGAGAGAACAGAACCTGAGGGCCCTTTTCAGCTATAT
CACGAGGAGGAATGTGTAGTGGATTCTCCTGAGCACGAAACACACGCAACTTTGTAAGCA
TTTTGAAGGAGTCAGGTAGAACACGGATCTGATTATTGCTTATATCAAGCTCTTCAAGCA
TCTCAAGGTTGCCTATTGATCTCGGGAGCGATACCATGTCAGCGAAATTGTTTCCAATAT
TCAGTTTCACAAGTGTAGTGGCAAAACACAAACTCTCTGGCACTGACTCGAGCTCGTTGA
AACTCACATCCAGCTCTTTGAGGCTAGCTAAAGAAGACATCGTCGTAGGTAACTGCCTGA
TGTTATTGTAACGCACAGACAAGATCTCTAATGTAGTAATCTTCCCAATAGCTTCTGGAA
GAGCCTTTAGTTTGTTATAATCTGCACGGAGTTCTATCAGAGAAGAACATCCACCAATAG
AATATGGGATCTCTTCGATATCATTGTTCTCAACG

Last Updated on 06.12.2018 16:11