Sequences from confirmation amplicon


Confirmation sequences for line 960B07 and hit At5g04120 are shown.
Line ID 960B07
Locus At5g04120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1120297
Primers used for PCR 94ra - 8474, show primer details
   
Sequence Name 39-K116491-0022-960-B07C-94ra
GenBank Accession LN503486 [GenBank]
Sequencing primer gene specific primer, 94ra: 5'-AATACCAATACCCCTTCCGATAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K116491-0022-960-B07C-94ra
ACTCGATTATTGCACAAATGTTTATTATTTTATTCGTAATATATACTAAGTTTTCACGTT
TATAGATATATCATAATGTTATATAGTATGCACGTCTTAAAATTCTAACTAGTTCAAAGA
ATGTCTTAAAATTCTTTAGTGTTTTATCGTCAATATACCAACCACATACAAAAATATTTG
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAATA
   
Sequence Name 07-K116490-0022-960-B07C-8409
GenBank Accession LN489434 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K116490-0022-960-B07C-8409
GCATTTCCAATATTTTTGTATGTGGTTGGTATATTGACGATAAAACACTAAAGAATTTTA
AGACATTCTTTGAACTAGTTAGAATTTTAAGACGTGCATACTATATAACATTATGATATA
TCTATAAACGTGAAAACTTAGTATATATTACGAATAAAATAATAAACATTTGTGCAATTA
ATCGAGTTTTGAAAAAAATACCATTCATGACCCATGTTATCGGAAGGGGTATTGGTATTA

Last Updated on 06.12.2018 16:11