Sequences from confirmation amplicon


Confirmation sequences for line 962C08 and hit At1g66920 are shown.
Line ID 962C08
Locus At1g66920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24966226
Primers used for PCR 21ow - 8474, show primer details
   
Sequence Name 60-K115005-0022-962-C08C-21ow
GenBank Accession LN510333 [GenBank]
Sequencing primer gene specific primer, 21ow: 5'-CATCTTCTTCGCTTCTATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K115005-0022-962-C08C-21ow
CCATTCGGGAAAGTCATTGAGCTTGTGCTAGATGTAGTGTCCTCGGTTGATGTTTTGTTC
CTAGCTCCTATTATATCAAGAACCAACATTCCATAGCTATACACATCTGACTTGTGGGAG
ACGCTACCGTACACTCTCGAAAACACTTCAGGTGCAATGTACCCTATCGTTCCTCTTGTG
TCCATTAGTGACAAGATACTTTCTTTCCTCTCGCATAGCTTAGCAAGGCCAAAGTCTGAT
ACTTTCGGGGAAAGATTGTCATCTAAGAGTACATTCTGTGGTTTTATGTCGAAATGTACA
ATCCTTGTTCTGCAACCATGGTGTAAGTACTCTAGACCACGAGCAACGCCTAACGCGATC
CCATATAGTTCCCTCCAGTCCATAGTTGATGACTTCTTGCTTGAGATGAACTTATCAAGA
GACCCGTTTTCCATAAATTCGTAAATAATTGCTCTCTTGTAACCTTCAGAGCAGAATCCT
AGGAGGGTAACAATGTTGACATGTGAAGTTTGGCTCATGCTTGCAACTTCATTGATAAAG
TCTACAATTCAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 28-K115004-0022-962-C08C-8409
GenBank Accession LN498971 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K115004-0022-962-C08C-8409
ATTTTTGATTGTAGACTTTATCAATGAAGTTGCAAGCATGAGCCAAACTTCACATGTCAA
CATTGTTACCCTCCTAGGATTCTGCTCTGAAGGTTACAAGAGAGCAATTATTTACGAATT
TATGGAAAACGGGTCTCTTGATAAGTTCATCTCAAGCAAGAAGTCATCAACTATGGACTG
GAGGGAACTATATGGGATCGCGTTAGGCGTTGCTCGTGGTCTAGAGTACTTACACCATGG
TTGCAGAACAAGGATTGTACATTTCGACATAAAACCACAGAATGTACTCTTAGATGACAA
TCTTTCCCCGAAAGTATCAGACTTTGGCCTTGCTAAGCTATGCGAGAGGAAAGAAAGTAT
CTTGTCACTAATGGACACAAGAGGAACGATAGGGTACATTGCACCTGAAGTGTTTTCGAG
AGTGTACGGTAGCGTCTCCCACAAGTCAGATGTGTATAGCTATGGAATGTTGGTTCTTGA
TATAATAGGAGCTAGGAACAAAACATCAACCGAGGACACTACATCTAGCACAAGCTCAAT
GTACTTTCCCGAATGGATATATAAGGATCTTGAGAAGGGAGACAATGGAAGGCTTATCGT
GAATAGAAGCGAANGAAAGATG

Last Updated on 06.12.2018 16:11