Sequences from confirmation amplicon


Confirmation sequences for line 963D01 and hit At5g45130 are shown.
Line ID 963D01
Locus At5g45130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18245987
Primers used for PCR AJ32 - 8474, show primer details
   
Sequence Name 40-K107201-0022-963-D01C-AJ32
GenBank Accession LN503726 [GenBank]
Sequencing primer gene specific primer, AJ32: 5'-CATATTACAGCATTCCAACAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K107201-0022-963-D01C-AJ32
TTGGAATTAGTATACAAAGATAATAAGCCTATAAATCTGAGCATTCACTTAAAACATAAA
CGAAAGATAAGCACATTTTTTTCGGACTAAGTTTTGTTTTCTAATTACAAAAGAGATATA
TATATATAAATCTCTCCAAATACAAATATCTTCAGATTTCACAAAGGCAGAATAAGTATC
ATCATGATATACACTGTTGTTGTTGTCAATGCAAGCAAAGTAAAGTAACAAAGACGAAGA
AGAAGCAAACACATGAAAGTCCAGATGTTCTCTACTATGTGACTAACCAAATGAGATCTC
TCTTCAGGTACGAATCTAAGCACAACACGATGAACTCACTGCCGTAGCCCCTGGCCCGTT
TGGGAGAACCATTCCTGTCGGGTTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATT
   
Sequence Name 36-K107200-0022-963-D01C-8409
GenBank Accession LN502245 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K107200-0022-963-D01C-8409
ATTTACAATTGAATTATCCTGAAAACCCGACAGGAATGGTTCTCCCAAACGGGCCAGGGG
CTACGGCAGTGAGTTCATCGTGTTGTGCTTAGATTCGTACCTGAAGAGAGATCTCATTTG
GTTAGTCACATAGTAGAGAACATCTGGACTTTCATGTGTTTGCTTCTTCTTCGTCTTTGT
TACTTTACTTTGCTTGCATTGACAACAACAACAGTGTATATCATGATGATACTTATTCTG
CCTTTGTGAAATCTGAAGATATTTGTATTTGGAGAGATTTATATATATATATCTCTTTTG
TAATTAGAAAACAAAACTTAGTCCGAAAAAAATGTGCTTATCTTTCGTTTATGTTTTAAG
TGAATGCTCAGATTTATAGGCTTATTATCTTTTGTATTACTTAAATCTCCAAAATGTTTT
TTCCATTGTTGGAATGCGNTAATATG

Last Updated on 06.12.2018 16:11