Sequences from confirmation amplicon


Confirmation sequences for line 964A01 and hit At5g44770 are shown.
Line ID 964A01
Locus At5g44770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18066155
Primers used for PCR 04rr - 8474, show primer details
   
Sequence Name 58-K116162-0022-964-A01C-04rr
GenBank Accession LN509798 [GenBank]
Sequencing primer gene specific primer, 04rr: 5'-TCAATGATGATCCATGCTACAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K116162-0022-964-A01C-04rr
TCGAAAGAAGCCCATATTTTGCACAACCATAAAATTAGCCTACGGGCTCATAAGGATGAC
GTTTATGTAGATAAATGCGGAGCTTGTAAAACTTATTCTAATGGTTTCAAGTACGAGTGT
CACGACAAAAGCAGCTGTAGAGGTAATATAAAGTATGATATCCGTTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 29-K116161-0022-964-A01C-8409
GenBank Accession LN499437 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116161-0022-964-A01C-8409
CATTTCACGGATATCATACTTTATATTACCTCTACAGCTGCTTTTGTCGTGACACTCGTA
CTTGAAACCATTAGAATAAGTTTTACAAGCTCCGCATTTATCTACATAAACGTCATCCTT
ATGAGCCCGTAGGCTAATTTTATGGTTGTGCAAAAAATGGGCTTTCTTTCGAGGAAGACT
AGCACATCCTTTGTGAAGGCAGAAATCACATTCCACGCACTTGTAGCATGGATCATCATT
GAA

Last Updated on 06.12.2018 16:11