Sequences from confirmation amplicon


Confirmation sequences for line 964B06 and hit At1g19540 are shown.
Line ID 964B06
Locus At1g19540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6767074
Primers used for PCR 72sm - 8474, show primer details
   
Sequence Name 57-K116569-0022-964-B06C-72sm
GenBank Accession LN509521 [GenBank]
Sequencing primer gene specific primer, 72sm: 5'-TGTTGTAATGCTGTTCAAGGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K116569-0022-964-B06C-72sm
TTTATGTATGTATTTGTATCTATCAAATAAGGAGACTAGCTTGAAATAAGATTTGCGTCC
ATATCGAAACTCGAATTAACAGTAGCTACACACACAAAATAATGGACGAAGTTTTTTTCA
GATAAACCGGTTAAGAAACTCATCAACACTAGTGTACTTGACTTCAGGGTAAAGCTCGGA
AGCCTCAACTCCAAAAGAAGGATCTATAGTGAAGGAGGTAAAGTCACTCTTCACAAGGAT
TGTATGGATCAGACCCACCAAAAAATCCATTGGAGGCTTACTCTCTGCAAATGAGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGCTTA
TTATAAT
   
Sequence Name 28-K116568-0022-964-B06C-8409
GenBank Accession LN499004 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116568-0022-964-B06C-8409
TCTTTGCAGAGAGTAAGCCTCCATGGATTTTTTGGTGGGTCTGATCCATACAATCCTTGT
GAAGAGTGACTTTACCTCCTTCACTATAGATCCTTCTTTTGGAGTTGAGGCTTCCGAGCT
TTACCCTGAAGTCAAGTACACTAGTGTTGATGAGTTTCTTAACCGGTTTATCTGAAAAAA
ACTTCGTCCATTATTTTGTGTGTGTAGCTACTGTTAATTCGAGTTTCGATATGGACGCAA
ATCTTATTTCAAGCTAGTCTCCTTATTTGATAGATACAAATACATAACATAAAAGGTCCA
AGTACATTATTTGACTGATATAAGAAAGAGACCTTGAACAGCATTACAAC

Last Updated on 06.12.2018 16:11