Sequences from confirmation amplicon


Confirmation sequences for line 965D08 and hit At5g13000 are shown.
Line ID 965D08
Locus At5g13000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4121480
Primers used for PCR BA38 - 8474, show primer details
   
Sequence Name 38-K104830-0022-965-D08C-BA38
GenBank Accession LN502969 [GenBank]
Sequencing primer gene specific primer, BA38: 5'-TTAGACATACCGGTCAGCCTTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K104830-0022-965-D08C-BA38
TTCTTAAGTAGTGTTGATAAAGCTTTGCATTTCACGGGCATCACTTTTCTTAACCCTGCC
CATCAGTGTTGGATCATGTTCCTGCAAAAAAATCAAAGCAACAGTAGACCACATTTGTCA
AAGAAAAGTACTAAAACCAAGATTCCTTCTAATAGCTATCCAAATCTGCCACATGCAAGA
ATCAAGCAGAGTATAAAGAATGCTCACTCTTTCAAGACGCTGTAGAAGTGCAGTCTTAAA
CTGCCGAACACCTCTTCCACTGGAGGTAGGATCCAATCTATGTGCTTTCTCAAATGCATA
AAACCGACCTAAGGGAGAAAGCAGAACCAAAAACCAGATAAACAACCAAATGACAGAGAA
CCAAATTAAGAAATAAGTTTTAGAAGAACTTACAGAGATACGCAACCCTAGGATTACTAG
ATTCAACTTCATTAGCAACCCGAAGAATGGGGGCAATCTCAACAAGAGATGATGGAACAA
CTTCACTATCGAATGACTCTCCAAGATTACCAGCAGTCTGAGTTCGTATAATCCGCCGCT
GCTGAGGCTGAGACGGCCCTTGATCAGGACCTCCTCTCGTAGCAGACATTTTGTCTACCC
TATAACCTCCAGGTTTTAAATCGTTTTAAGGTTTCCTCAGCCTCCCTGAAAACAAACAAA
AGTCATTAGACGAAGATGGTTAAAAATCAACAGTGACGAACAGAGATGCAACATTGAGAA
GAACATACAGAGAAACGGAGATCCAGAAACCCTAACAATGGCGGAGCAAAGGAGCCACAA
CTTTTTAAGCTACTTAGCTGCTAAGTACGCAAAGTGAAAGGGAGAGAGAGAGAGCAGTCT
GAAATGAATGTTTTTTTGTATTTATCTGCTGAGGCTGAGACGGCCCTTGATCGATCCTTT
ATAGGGTTTCGCAATGAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAGAAAGAGTAATACCAATT
TTTTT
   
Sequence Name 53-K104831-0022-965-D08C-8409
GenBank Accession LN508147 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K104831-0022-965-D08C-8409
GCCATTTACAATTGAATATATCCTGTTCATTGCGAAACCCTATAAAGGATCGATCAAGGG
CCGTCTCAGCCTCAGCAGATAAATACAAAAAAACATTCATTTCAGACTGCTCTCTCTCTC
TCCCTTTCACTTTGCGTACTTAGCAGCTAAGTAGCTTAAAAAGTTGTGGCTCCTTTGCTC
CGCCATTGTTAGGGTTTCTGGATCTCCGTTTCTCTGTATGTTCTTCTCAATGTTGCATCT
CTGTTCGTCACTGTTGATTTTTAACCATCTTCGTCTAATGACTTTTGTTTGTTTTCAGGG
AGGCTGAGGAAACCTTAAAACGATTTAAAACCTGGAGGTTATAGGGTAGACAAAATGTCT
GCTACGAGAGGAGGTCCTGATCAAGGGCCGTCTCAGCCTCAGCAGCGGCGGATTATACGA
ACTCAGACTGCTGGTAATCTTGGAGAGTCATTCGATAGTGAAGTTGTTCCATCATCTCTT
GTTGAGATTGCCCCCATTCTTCGGGTTGCTAATGAAGTTGAATCTAGTAATCCTAGGGTT
GCGTATCTCTGTAAGTTCTTCTAAAACTTATTTCTTAATTTGGTTCTCTGTCATTTGGTT
GTTTATCTGGTTTTTGGTTCTGCTTTCTCCCTTAGGTCGGTTTTATGCATTTGAGAAAGC
ACATAGATTGGATCCTACCTCCAGTGGAAGAGGTGTTCGGCAGTTTAAGACTGCACTTCT
ACAGCGTCTTGAAAGAGTGAGCATTCTTTATACTCTGCTTGATTCTTGCATGTGGCAGAT
TTGGATAGCTATTAGAAGGAATCTTGGTTTTAGTACTTTTCTTTGACAAATGTGGTCTAC
TGTTGCTTTGATTTTTTTGCAGGAACATGATCCAACACTGATGGGCAGGGTTAAGAAAAG
TGATGCCCGTGAAATGCAAAGCTTTTACAACACTACTATAAGAAGTATTTTCAAGCTTTG
CACAATGCTGCTG

Last Updated on 06.12.2018 16:11