SimpleSearch - Line and FST details


Line specific information

 
Line ID 965D08
Vector Used pAC161
Line Availability available as T3 set from NASC (N492588)
Segregation Analysis 50:50:38
Confirmed for Hit At5g13000
Parent of DUPLO pair none
Parent of pair(s) 5543, 5552, 5581, 5659, 5664, 92355, 92364, 92365, 92366
Note

Gene hit At2g42770

 
Sequence (A. th genome BLAST matches underlined) 
>60-K113265-0022-965-D08-8409
ACCATTATTATCTTTCTTTCAAAACANGTATATNGNTGNTCAAATNTNAAAANNNNNNTT
NTNTNTNNNTGNNNNNNNNTGTGGNGGGGNNNANATTTTTGNGCTTGGAACCNNTTNTCA
NAANNATGCTCTTCCTACTCTTCTTTATGGGGAGCTACC
GenBank Accession HE667272 [GenBank]
Graphic View Graphic view of gene At2g42770
Predicted Position of Insertion Chr2:17799108 - go to primer design
BLAST e Value 3e-05
Hit Clone Code (BAC ID) F7D19
Hit Gene Code At2g42770 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Peroxisomal membrane 22 kDa (Mpv17/PMP22) family protein
Insertion Classification CDSi
Confirmation Status failed

Gene hit At5g13000

 
Sequence (A. th genome BLAST matches underlined) 
>60-K113264-0022-965-D08-8409
TTTCACTGTATGTTCTTCTCAGTGTTGCATCTCTGTTCGTCACGGTTGATTTTTGCCCAT
CTTCGACTAATGACTTTTGTTTGTTTTCAGGGAGGCTGAGGAAACCTTAAAACGATTTAA
AACCTGAAGGTTATAGGGTAGACAAAATGTCTGCTACGAGAGGAGGTCCTGATCAAGGGC
CGTCTCAGCCTCAGCAGCGGCGGATTATACGAACTCAGACTGCTGGTAATCTTGGAGAGT
CATTCGATAGTGAAGTTGTTCCATCATCTCTTGTTGAGATTGCCCCCATTCTTCGGGTTG
CTAATGAAGTTGAATCTAACCTGCCCGGCCG
GenBank Accession HE667271 [GenBank]
Graphic View Graphic view of gene At5g13000
Predicted Position of Insertion Chr5:4121529 - go to primer design
BLAST e Value 5e-163
Hit Clone Code (BAC ID) T24H18
Hit Gene Code At5g13000 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation glucan synthase-like 12
Insertion Classification Promoter
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details
Other FSTs Supporting this Hit CU458522 [GenBank]


Last Updated on 10.06.2021 13:37