Sequences from confirmation amplicon


Confirmation sequences for line 966E06 and hit At4g16350 are shown.
Line ID 966E06
Locus At4g16350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9242646
Primers used for PCR 03mz - 8474, show primer details
   
Sequence Name 39-K114277-0022-966-E06C-03mz
GenBank Accession LN503436 [GenBank]
Sequencing primer gene specific primer, 03mz: 5'-TTTGCACACTCCAACAGATAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K114277-0022-966-E06C-03mz
CTGCTTTCAGAACGTATTTATTAAAAATAAAGAGGAGGGTTGTAATAGAGTAATGGAGAA
GAAGATAAGAATCAAGTATGAATGATCTTGTAAAGGGTTTTGGTCTTTCTTCACATCCCT
GATCATCCATCCAGCTCACTAGGAGTGTCTGTCACTATAGTGTGAAATACAAAATTTGGA
AACGTCTTTGTTACGTCTCTACACAAAACACAACATACAAAAACATGTTAGTCCCGCACG
AAGAAGATAATAAGGCAAATAACAAGCCGAGAAAAAGAAAGAGTAAATATGGCTTCATGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 09-K114276-0022-966-E06C-8409
GenBank Accession LN490279 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114276-0022-966-E06C-8409
GGAAGAAGCCATTTAATGAAGCCATATTTACTCTTTCTTTTTCTCGGCTTGTTATTTGCC
TTATTATCTTCTTCGTGCGGGACTAACATGTTTTTGTATGTTGTGTTTTGTGTAGAGACG
TAACAAAGACGTTTCCAAATTTTGTATTTCACACTATAGTGACAGACACTCCTAGTGAGC
TGGATGGATGATCAGGGATGTGAAGAAAGACCAAAACCCTTTACAAGATCATTCATACTT
GATTCTTATCTTCTTCTCCATTACTCTATTACAACCCTCCTCTTTATTTTTAATAAATAC
GTTCTGAAAGCAGCATTACCTCACATGTTTGTGTATCCGCAAACTTGTTCGGTTTATCTT
ATCTGTTGAGATGTGCAA

Last Updated on 06.12.2018 16:11