Sequences from confirmation amplicon


Confirmation sequences for line 967G02 and hit At2g19580 are shown.
Line ID 967G02
Locus At2g19580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8474241
Primers used for PCR 20jk - 8474, show primer details
   
Sequence Name 96-K113193-0022-967-G02C-20jk
GenBank Accession LN515391 [GenBank]
Sequencing primer gene specific primer, 20jk: 5'-GGTGGTTCTTATATTTGCATTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K113193-0022-967-G02C-20jk
GGTTCGGGTAGAGGTTAAAAGAGTATAGGCTTGAAGGGTTCTCGAATTGGCTTAAGGAGA
ACGTTGTGGATTCCAAGAACTGGGGAAGGCTAAGGGCTTGTTTGGCTGATACTAATGTTT
GTCCTAAACTCAACCAAGAATTCATCACCGCCGATCAGTTCTTCTCCTCCTCTAAGATCA
CTCCTCTCCAGGCATGTTCTTTCTTCTTTTATTCTCTTTTATAAAAAGAACCCCGAAATT
TCTAGTTTCCGGTTTAGGTTTGGTTTAGAGGTAAATCTAGACTAAATATCGGTTATGCAA
CTATGCCAACGAATCATTGTGGGTTTAATTAGATATTATTAGATGATTAATGGAGATAAG
ATACGAAGTAAAGAGAATGTTTTGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 59-K113192-0022-967-G02C-8409
GenBank Accession LN510050 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K113192-0022-967-G02C-8409
TTACAATTGAATATACAAAACATTCTCTTTACTTCGTATCTTATCTCCATTAATCATCTA
ATAATATCTAATTAAACCCACAATGATTCGTTGGCATAGTTGCATAACCGATATTTAGTC
TAGATTTACCTCTAAACCAAACCTAAACCGGAAACTAGAAATTTCGGGGTTCTTTTTATA
AAAGAGAATAAAAGAAGAAAGAACATGCCTGGAGAGGAGTGATCTTAGAGGAGGAGAAGA
ACTGATCGGCGGTGATGAATTCTTGGTTGAGTTTAGGACAAACATTAGTATCAGCCAAAC
AAGCCCTTAGCCTTCCCCAGTTCTTGGAATCCACAACGTTCTCCTTAAGCCAATTCGAGA
ACCCTTCAAGCCTATACTCTTTATAACCTCTACCCGGAACCCGATACGATCCATCGGGCC
GGGTCACGACGAATGCAAATANTAGAACCAC

Last Updated on 06.12.2018 16:11