Sequences from confirmation amplicon


Confirmation sequences for line 968G03 and hit At3g14640 are shown.
Line ID 968G03
Locus At3g14640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4921715
Primers used for PCR 66xk - 8474, show primer details
   
Sequence Name 59-K119407-0022-968-G03C-66xk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 66xk: 5'-TTACAGATTGTGAACCATGATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K119407-0022-968-G03C-66xk
TTTGTGTAGTATCTCCCTACGAAGAGTAATAGAAGGATGAAAGCAGCAGCTAGAGAAATC
CAAGTTATACTGAAAGGGATCGTTAACAAAAGGTTAAGGGCGAGAGAAGCTGGGAAAGCA
GCACCAAACGACGATTTGTTGGGAATACTTCTTGAATCAAATTTGGGGCAAGCCAAAGGA
AATGGAATGAGTACTGAGGATGTGATGGAGGAGTGCAAGTTGTTCTATTTCGCCGGGCAA
GAGACAACTTCGGTACTTCTGGTCTGGGCAATGGTGCTGTTAAGCCACCACCAAGATTGG
CAGGCTCGTGCACGAGAGGAAGTGAAGCAAGTTTTTGGCGATAAAGAACCTGATACAGAA
TGTCTTAGCCAGCTCAAAGTTGTAAGTATAAACCAAATCATAAACCTTCAGGTGACTTTA
GTACTTTTATTGTGTTTAGATGGTTTTGCTGAATGTTTCCAACAGATGACAATGATATTA
TATGAGGTTCTCAGGCTATATCCTCCTGTAACTCACCTGACCCGAGCGATTGACAAAGAG
ATGAAGCTAGGTGACTTGACACTACCAGCCGGTGTTCATATCAGTCTACCTATAATGCTA
GTCCAACGCGACCCCATGCTGTGGGGTACTGATGCAGCAGAATTTAAGCCTGAGAGATTC
AAAGACGGTCTTTCAAAGGCAACAAAGAGCCAAGTCTCCTTCTTTCCATTTGCATGGGGA
CCGAGGATATGCATCGGCCAGAATTTTGCCATGTTAGAGGCAAAGATGGCAATGGCGTTG
ATTCTACAGACATTCACCTTCGAGCTTTCTCCTTCCTATTTTATATTTTGGTGGTTTGGA
CAATTATTTTGGTGGATATTTCATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAGAAAGAGTAATTACCATTTTTTTTTC
   
Sequence Name 19-K119406-0022-968-G03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119406-0022-968-G03C-8409
ATTTACAATTGAATATCCACCAAAATAATTGTCCAAACCACCAAAATATAAAATAGGAAG
GAGAAAGCTCGAAGGTGAATGTCTGTAGAATCAACGCCATTGCCATCTTTGCCTCTAACA
TGGCAAAATTCTGGCCGATGCATATCCTCGGTCCCCATGCAAATGGAAAGAAGGAGACTT
GGCTCTTTGTTGCCTTTGAAAGACCGTCTTTGAATCTCTCAGGCTTAAATTCTGCTGCAT
CAGTACCCCACAGCATGGGGTCGCGTTGGACTAGCATTATAGGTAGACTGATATGAACAC
CGGCTGGTAGTGTCAAGTCACCTAGCTTCATCTCTTTGTCAATCGCTCGGGTCAGGTGAG
TTACAGGAGGATATAGCCTGAGAACCTCATATAATATCATTGTCATCTGTTGGAAACATT
CAGCAAAACCATCTAAACACAATAAAAGTACTAAAGTCACCTGAAGGTTTATGATTTGGT
TTATACTTACAACTTTGAGCTGGCTAAGACATTCTGTATCAGGTTCTTTATCGCCAAAAA
CTTGCTTCACTTCCTCTCGTGCACGAGCCTGCCAATCTTGGTGGTGGCTTAACAGCACCA
TTGCCCAGACCAGAAGTACCGAAGTTGTCTCTTGCCCGGCGAAATAGAACAACTTGCACT
CCTCCATCACATCCTCAGTACTCATTCCATTTCCTTTGGCTTGCCCCAAATTTGATTCAA
GAAGTATTCCCAACAAATCGTCGTTTGGTGCTGCTTTCCCAGCTTCTCTCGCCCTTAACC
TTTTGTTAACGATCCCTTTCAGTATAACTTGGATTTCTCTAGCTGCTGCTTTCATCCTTC
TATTACTCTTCGTAGGGAGATAACTACACAAACAGAAGACAGAGAGAATATGAGTATATG
ACACATCAT

Last Updated on 06.12.2018 16:11