Sequences from confirmation amplicon


Confirmation sequences for line 969E09 and hit At3g10330 are shown.
Line ID 969E09
Locus At3g10330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3200126
Primers used for PCR 11ht - 8474, show primer details
   
Sequence Name 49-K112586-0022-969-E09C-11ht
GenBank Accession LN506892 [GenBank]
Sequencing primer gene specific primer, 11ht: 5'-AAGCTAGATTCCAAGAAACCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K112586-0022-969-E09C-11ht
GCTTTCAGTAGAAGCCATAAGATTGCAAGAGAAAGAGAGTAAGTACCTTGATGGTCGCAA
CAAGTCCCAACCTGGAGCAACAACAAGGTTCATAGAATTAGTAAATGGCATTTGTCAAAC
AACCTAACTTAGAGTAAAAACAGCTCACAATAACATTTCATAACGCATTGAACCAATTAT
TATACCACGGATAATGAGCAAGAATCAAGTTTTCTCATTTCCCAGATATCTAAAAATAGA
TAGTTTCTATTTTCAGTGACATACATTTGCTTATACCCATCATAAGATTCATCTAGAATT
CTAGGTATTACAATTCTTCTACATACTAAAATTCTCAAAATTGCTGAAATTGAGCTTCAA
GAAAACACTGAATTGTAGAAATTGGGCTTAAAAAACAAACCCTAGAATCGAAAAACGAAC
CTATCAGCCATGGTAGCGATAGTCTTGAAAGCAACAATAAGGCCACGATCAGGATTAGAT
CCACGATTCTGCCATCGACCAAGAGAAGAAGAGAGGAAATCGCCNGAGGATCCATTAGGT
TTAGAAATGACAGTAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCA
   
Sequence Name 23-K112585-0022-969-E09C-8409
GenBank Accession LN496622 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K112585-0022-969-E09C-8409
CATTTACACTGTCATTTCTAAACCTAATGGATCCTCTGGCGATTTCCTCTCTTCTTCTCT
TGGTCGATGGCAGAATCGTGGATCTAATCCTGATCGTGGCCTTATTGTTGCTTTCAAGAC
TATCGCTACCATGGCTGATAGGTTCGTTTTTCGATTCTAGGGTTTGTTTTTTAAGCCCAA
TTTCTACAATTCAGTGTTTTCTTGAAGCTCAATTTCAGCAATTTTGAGAATTTTAGTATG
TAGAAGAATTGTAATACCTAGAATTCTAGATGAATCTTATGATGGGTATAAGCAAATGTA
TGTCACTGAAAATAGAAACTATCTATTTTTAGATATCTGGGAAATGAGAAAACTTGATTC
TTGCTCATTATCCGTGGTATAATAATTGGTTCAATGCGTTATGAAATGTTATTGTGAGCT
GTTTTTACTCTAAGTTAGGTTGTTTGACAAATGCCATTTACTAATTCTATGAACCTTGTT
GTTGCTCCAGGTTGGGACTTGTTGCGACCATCAAGGTACTTACTCTCTTTCTCTTTGCAA
TCTTATGGCTTCTACTTGTAAAGCTTTCTGTTTTGTTAAGTTGCTGATGGTTTCTTGGAA
TCTAGCT

Last Updated on 06.12.2018 16:11