Sequences from confirmation amplicon


Confirmation sequences for line 970F11 and hit At3g10200 are shown.
Line ID 970F11
Locus At3g10200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3159015
Primers used for PCR 56fr - 8474, show primer details
   
Sequence Name 30-K111563-0022-970-F11C-56fr
GenBank Accession LN499778 [GenBank]
Sequencing primer gene specific primer, 56fr: 5'-TGACTAATGAAACAGGTGACTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K111563-0022-970-F11C-56fr
GCTGTGGAGTTGCGAGCTTTGCGGCTTATCTTCTTCCCTTAGGTATACAGACAATTTCAT
TTGCTCCCAAAGATGGTCATGAAAACCAGATTCAGTTTGCATTGGAGAGAGGCATTGGTG
CAATGATATCCGCGGTTGCCACCAAACAATTGCCATATCCTGCAGCCTCGTTTGAGATGG
TTCATTGTTCGAGATGTCGTGTTGACTGGCATACAAATGGTATGTATGTATTCTTGTGTT
GAATTTGGTTATAAGAAGTTGGTTGATAATGCTTCCGCTTGGTTCCTCAGATGGCATCTT
ACTTAAAGAAGTTCATCGTCTTCTCCGACCAAATGGATTCTTCGTGTATTCATCACCACC
AGCATATAGAAAGGATAAAGAGTATCCTATGATTTGGGATAAGTTGGTCAATCTAACTAG
TGCAATGTGCTGGAAGCTTATTTCCCGGAAGGTACAGACTGCAATATGGATTAAAGAAGA
GAAGGAGGTTTGTCTTAAGCAGAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGATTA
   
Sequence Name 14-K111556w-0022-970-F11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K111556w-0022-970-F11C-8409
ATTTCATTGAAATATCCTGCTCTGCTTAAGACAACCTCCTTCTCTTCTTTATCCATATTG
CAGTCTGTACCTTCCGGGAAATAAGCTTCCAGCACATTGCACTAGTTAGATTGACCAACT
TATCCCAAATCATAGGATACTCTTTATCCTTTCTATATGCTGGTGGTGATGAATACACGA
AGAATCCATTTGGTCGGAGAAGACGATGAACTTCTTTAAGTAAGATGCCATCTGAGGAAC
CAAGCGGAAGCATTATCAACCAACTTCTTATAACCAAATTCAACACAAGAATACATACAT
ACCATTTGTATGCCAGTCAACACGACATCTCGAACAATGAACCATCTCAAACGAGGCTGC
AGGATATGGCAATTGTTTGGTGGCAACCGCGGATATCATTGCACCAATGCCTCTCTCCAA
TGCAAACTGAATCTGGTTTTCATGACCATCTTTGGGAGCAAATGAAATTGTCTGTATACC
TAAGGGAAGAAGATAAGCCGCAAAGCTCGCAACTCCACAGCCAACATCAAGAACTTGTAC
CACCCCAGCTGAACGCAAGTCACCTGTTTCATTAGTC

Last Updated on 06.12.2018 16:11