Sequences from confirmation amplicon


Confirmation sequences for line 970H03 and hit At2g47630 are shown.
Line ID 970H03
Locus At2g47630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19536049
Primers used for PCR 30sw - 8474, show primer details
   
Sequence Name 40-K116770-0022-970-H03C-30sw
GenBank Accession LN503840 [GenBank]
Sequencing primer gene specific primer, 30sw: 5'-TACCTGCATTTACACACACCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K116770-0022-970-H03C-30sw
GTTGGTATACACTATACAGACCTCTTCTCGCTTGACGAGGTCCTTGAAAGCAGCGTCAAT
CACGTCTTTTGTTGGAACTATTTTCCATTTAGGTATGATTTCTTCGACTCTAGTTAACAG
ATTAATCACTATAGGGTGTGGCTTCACCTTCTCAGATATCTGCATATTTAAACATAACAA
TATACTTTGCATTTCAATATTATTATCACCTACGACTCTGCAATCTAAAATTATAGTTGC
AGGTTTTTTACATGTATATACATGGATCAGCAATGAGTATGATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCTACAAGGTTATTAT
   
Sequence Name 16-K116769-0022-970-H03C-8409
GenBank Accession LN493595 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116769-0022-970-H03C-8409
TACTCATTGCTGATCCAGNTATATACATGTAAAAAACCTGCAACTATAATTTTAGATTGC
AGAGTCGTAGGTGATAATAATATTGAAATGCAAAGTATATTGTTATGTTTAAATATGCAG
ATATCTGAGAAGGTGAAGCCACACCCTATAGTGATTAATCTGTTAACTAGAGTCGAAGAA
ATCATACCTAAATGGAAAATAGTTCCAACAAAAGACGTGATTGACGCTGCTTTCAAGGAC
CTCGTCAAGCGAGAAGAGGTCTGTATAGTGTATACCAACCCTCTGATCACCATCGAAATT
GGTTATATGTAAATAATTTGGTGTGTGTAAATGCAGGTA

Last Updated on 06.12.2018 16:11