Sequences from confirmation amplicon


Confirmation sequences for line 971B10 and hit At2g29360 are shown.
Line ID 971B10
Locus At2g29360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12604914
Primers used for PCR 20qb - 8474, show primer details
   
Sequence Name 54-K115550-0022-971-B10C-20qb
GenBank Accession LN508580 [GenBank]
Sequencing primer gene specific primer, 20qb: 5'-GCAGGTTATAACTTATCAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115550-0022-971-B10C-20qb
TACAATTATACTATAATTAAAAAAAATTAGTATACTTGATCGGTTAAAACACAAAGCATG
CATATATTGTGGAGAGTTGGATTACATTCCAAACGCCAATTTTTTTTGTTTGTTTGTGTA
ATTGTGTTTATGTTTACATATCTAAACAAATTGTAAATGTTAAAAAAATAACGTAAAATG
AAAACAATCGACGTCTTAAGGCAGAGGCTTGAAAGAGAAACCATTAACAGTGAAACCTCC
ATCAACACAAATGGTTTGACCTGTAATATAAGAAGCTGCAGGAAGACAAAGAAATGCCAC
AAGCGATGATACTTCATTTACTTCTCCAACACGTCCCATTGGTGGTCTACTCTCCACTTC
TTTTCTAAACTCCTCATTACTAAGACTCTGAGAGATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 27-K115549-0022-971-B10C-8409
GenBank Accession LN498531 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115549-0022-971-B10C-8409
ATTTACATTGAATATCTCTCAGAGTCTTAGTAATGAGGAGTTTAGAAAAGAAGTGGAGAG
TAGACCACCAATGGGACGTGTTGGAGAAGTAAATGAAGTATCATCGCTTGTGGCATTTCT
TTGTCTTCCTGCAGCTTCTTATATTACAGGTCAAACCATTTGTGTTGATGGAGGTTTCAC
TGTTAATGGTTTCTCTTTCAAGCCTCTGCCTTAAGACGTCGATTGTTTTCATTTTACGTT
ATTTTTTTAACATTTACAATTTGTTTAGATATGTAAACATAAACACAATTACACAAACAA
ACAAAAAAAATTGGCGTTTGGAATGTAATCCAACTCTCCACAATATATGCATGCTTTGTG
TTTTAACCGATCAAGTATACTAATTTTTTTTTAATTATAGTATAATTTGTTACTACGAAG
CCACCAACGTTCTTACATTCTCAAGACATTGATAAGTTATAACCT

Last Updated on 06.12.2018 16:11