Sequences from confirmation amplicon


Confirmation sequences for line 971D05 and hit At4g22212 are shown.
Line ID 971D05
Locus At4g22212 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11754050
Primers used for PCR 15ny - 8474, show primer details
   
Sequence Name 74-K114716-0022-971-D05C-15ny
GenBank Accession LN513125 [GenBank]
Sequencing primer gene specific primer, 15ny: 5'-ATACAGTTAGTGAGTATATTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K114716-0022-971-D05C-15ny
ATGCCTGGATAGTGGAATATTTTAACAATTATTATTCTCTTACGCGTGGAAAGTTTTAAA
TTACTAAAAAAGGTAAACTTGGATAATCATTTTATTGTAAAGATATTGACAATTTGACAT
AAAGTATTTTGATCTATGTACCCATTCCAATATATAAAATTGCATAGTGGTTTTTTCTTA
CAAAAAAAAAAAAGTAACATCTAGATCCCCTTTCCCTTGGAGTGCTATAAATTCAAACCC
CTTCCCTAAATAAATCACAAAGCGAGAAAAAAAAACAAATTGATCACCATTGGGAAGCTA
AAGGGTGAGCACATTTGTTATAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAA
TGAGTATAATGGTCAATATGGAAAAAAAAAAAAAGTAATTACCATTTTTTTTTCAATTCA
AAAATGTAAATTTCCCCACCAATTATAAAAAAA
   
Sequence Name 26-K114715-0022-971-D05C-8409
GenBank Accession LN498044 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114715-0022-971-D05C-8409
TTATAACAACTGTGCTCACCCTTAAGCTTCCCATTGCTGATCAGTTCGTCTTTCTTTCTC
GCTTTGTGATTTATTTAGGGAAGGAGTCTGAATTTATAGCACTCCAAGGGAAAGGGCATC
TAGATGTTACTCTTTTTTTTTTGTAAAAAAAAACCACTATGCAATTTTATATATGGAAAG
GGGTACATAAATCAAAATACTTTATGTCAAATTGTCAATATCTTTACAATAAAATGATTA
TCCAATTTTACCTTTTTTAGTAATTTAAAACTTTCCACCCGTAAAAAAATAATAATTGTT
AAAATATTTCCACTAATCCAGGCATCCACTCACCTACCTTTAATTTGGTCTATAGTATTG
AAATATACCCACTAACTGT

Last Updated on 06.12.2018 16:11