Sequences from confirmation amplicon


Confirmation sequences for line 971D12 and hit At5g17500 are shown.
Line ID 971D12
Locus At5g17500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5768823
Primers used for PCR 37pf - 8474, show primer details
   
Sequence Name 19-K115021-0022-971-D12C-37pf
GenBank Accession LN494881 [GenBank]
Sequencing primer gene specific primer, 37pf: 5'-GTTAACGGATGAAATATTTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115021-0022-971-D12C-37pf
TAATTACATCAAACTATATGTAATAATAATCTGGTTAAATTACAAGCAAGATTGATGAAT
ACCTGTGTGCGGTGGTTGAATGACGGAGAGCCTACGGAGATAAGTATAGTTATGAACATT
ATGCCAATTCGCATCTAACATACCGTAAGCTTCAACTACACCACGTTTGCCTTCGCGGAA
ATAATAAACTCCCGTAACCGCCCAAACCGCCCAATCCAAATCTTTCTCGGCCGCCCATGC
CAACATACAATTCATGTACCTAATGGTATACGGTTGATAAAAGTACTTTAAGCTTTGATT
GGTAAGCAAATATCTAAATGTAATTATAAAGGAAAGCCTTGGTCGAGAACCTGTTTCCTT
CAAGATCGCCACCTCTTTGATCGGTCCCAAACTCGCTCAAGAACAAAGGAAAGCCTTGGT
CGAGAACAAAACCGCCGGTACGACGCTCCTTAGAGAACATTTGGCTGCAAAAGTCGTTGA
CATTGTGTGACTTCCATTGACCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 06-K115020-0022-971-D12C-8409
GenBank Accession LN488932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115020-0022-971-D12C-8409
ATGTCACGACTTTTGCAGCCAATGTTCTCTAAGGAGCGTCGTACCGGCGGTTTTGTTCTC
GACCAAGGCTTTCCTTTGTTCTTGAGCGAGTTTGGGACCGATCAAAGAGGTGGCGATCTT
GAAGGAAACAGGTTCTCGACCAAGGCTTTCCTTTATAATTACATTTAGATATTTGCTTAC
CAATCAAAGCTTAAAGTACTTTTATCAACCGTATACCATTAGGTACATGAATTGTATGTT
GGCATGGGCGGCCGAGAAAGATTTGGATTGGGCGGTTTGGGCGGTTACGGGAGTTTATTA
TTTCCGCGAAGGCAAACGTGGTGTAGTTGAAGCTTACGGTATGTTAGATGCGAATTGGCA
TAATGTTCATAACTATACTTATCTCCGTAGGCTCTCCGTCATTCAACCACCGCACACAGG
TATTCATCAATCTTGCTTGTAATTTAACCAGATTATTATTACATATAGTTTGATGTAAAT
TTAATAAACAGGACCCGGTGTAAAGCACAATCATCACAAGAAAATATTTCATCCGTTAA

Last Updated on 06.12.2018 16:11