Sequences from confirmation amplicon


Confirmation sequences for line 971E11 and hit At3g26880 are shown.
Line ID 971E11
Locus At3g26880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9906647
Primers used for PCR 12ny - 8474, show primer details
   
Sequence Name 75-K114716-0022-971-E11C-12ny
GenBank Accession LN513287 [GenBank]
Sequencing primer gene specific primer, 12ny: 5'-GGAGATTTCTAGCTGATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K114716-0022-971-E11C-12ny
AACTTTATAGGTGTCTAGAATGACCCACTTAACGAAACTTGACATTTTTATCGAAAAGTG
AAACTAAAGTTTACGATTAAATGACGTTTCGAAAATTCATATTTTTTATCCCAAATTTTA
TAGTTTACATTTTCTTACATTTTTGCCATTTCTTTTTCTCTTTTCTTTTATTGTTTCAGT
AAAACTTATAGCCAATAAGTTTTATAGTAGCTAGGCAACGAATCCTTATGAAAATAGATT
CCATCATCTCTAGCTAACCAATTATACGATGATCCATCTTTGGCGAACAAACCTATAGCT
TGATATGCAGTAATACCTCTATAACGCTTATAATCAGGTCCTTTATATAAGTTGCACCAA
AATTCCGTCGTTCCCCAAAAGTTGGTCTTGAAGCTGAATGACATAACTTCTCCNATTTTG
AGATATTTTACACCAATATCGTTGTTTTTGGATTTACAATGCACCTTGAGGATATTCTTG
TTTTTGGGATTTAATTCATCAATAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCANAAATGTAGATATCCGCA
   
Sequence Name 27-K114715-0022-971-E11C-8409
GenBank Accession LN498509 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114715-0022-971-E11C-8409
TTGAATATATTATTGATGAATTAAATCCCAAAAACAAGAATATCCTCAAGGTGCATTGTA
AATCCAAAAACAACGATATTGGTGTAAAATATCTCAAAATTGGAGAAGTTATGTCATTCA
GCTTCAAGACCAACTTTTGGGGAACGACGGAATTTTGGTGCAACTTATATAAAGGACCTG
ATTATAAGCGTTATAGAGGTATTACTGCATATCAAGCTATAGGTTTGTTCGCCAAAGATG
GATCATCGTATAATTGGTTAGCTAGAGATGATGGAATCTATTTTCATAAGGATTCGTTGC
CTAGCTACTATAAAACTTATTGGCTATAAGTTTTACTGAAACAATAAAAGAAAAGAGAAA
AAGAAATGGCAAAAATGTAAGAAAATGTAAACTATAAAATTTGGGATAAAAAATATGAAT
TTTCGAAACGTCATTTAATCGTAAACTTTAGTTTCACTTTTCGATAAAAATGTCAAGTTT
CGTTAAGTGGGTCATTCTAGACACCTTATAAAGTTTTTGTTAATTAGACGTTTATGGGTT
TAACAATCA

Last Updated on 06.12.2018 16:11