Sequences from confirmation amplicon


Confirmation sequences for line 971G06 and hit At4g27550 are shown.
Line ID 971G06
Locus At4g27550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13756152
Primers used for PCR 35pf - 8474, show primer details
   
Sequence Name 70-K115259-0022-971-G06C-35pf
GenBank Accession LN512440 [GenBank]
Sequencing primer gene specific primer, 35pf: 5'-ATTGATCACACACTTCTTCCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K115259-0022-971-G06C-35pf
GAATGAATCATCATATTATTAACAAAAAAAAAGTTACCACAATCTTTAGCATTTTAACCA
AAAAAAATGTTTTAATATGATAATTAATGACATAATCTAGGTGGTCGTGTTTTATTAGTC
AAGATCAATTAATGATCATTGATTTATGGTTGTGTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 30-K115258-0022-971-G06C-8409
GenBank Accession LN499848 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K115258-0022-971-G06C-8409
CATTTCATTGAATATATCCTGACACAACCATAAATCAATGATCATTAATTGATCTTGACT
AATAAAACACGACCACCTAGATTATGTCATTAATTATCATATTAAAACATTTTTTTTGGT
TAAAATGCTAAAGATTGTGGTAACTTTTTTTTTGTTAATAATATGATGATTTCATTCTTG
GTTTTGGTTTTGTAGGGATGTATCCCAGTGTTTTTGGAAAAAGTGTGTGATCA

Last Updated on 06.12.2018 16:11