Sequences from confirmation amplicon


Confirmation sequences for line 973B12 and hit At4g34750 are shown.
Line ID 973B12
Locus At4g34750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16577574
Primers used for PCR 21hq - 8474, show primer details
   
Sequence Name 49-K112810-0022-973-B12C-21hq
GenBank Accession LN506897 [GenBank]
Sequencing primer gene specific primer, 21hq: 5'-CCCAAAAACAAAGTCAAAAATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K112810-0022-973-B12C-21hq
TATTAAACTTTCAAATTAATCTCTAACCAAGTTTCATCACATTCCGTTTGCAAATTTCAT
TAGCACACAGATTTTTCAGCAATCCCAGGAAGTAACGGACGAGATTCAACGTTGTTCTTC
GCCAATCCAACATGACTACACCTCCGGAGATCCTCAAGCGTCGCCGCCGGAGGATTACCT
CGTCCAGATGATGATGACGACTCACACCGAGTCACAATCGCAATAATATCCTCAAACAAC
GATTCATCACAAGGAATCGCTAACGGACCAACGTTAGCGAATCCATACTCTTCCTCAGCC
TCAGCTAGAAGCCGTCGGAAAATCGGATGGTTCAAATGCTTAGCACGGACAACGTATCTC
CGACGATTCTCACCGACGGAAACAGCAACGTGACCAGGAGGTACATCGGAAACAGGATCG
TTGTTGCTAGATCCGATGTGAGCTTTTTTCTGCCATTGTTTAAGCATCTGACGGATCCGA
ACAACGCTTCCGATCTTATTGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 24-K112809-0022-973-B12C-8409
GenBank Accession LN497064 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112809-0022-973-B12C-8409
TTGAACATAAGATCGGAAGCGTTGTTCGGATCCGTCAGATGCTTAAACAATGGCAGAAAA
AAGCTCACATCGGATCTAGCAACAACGATCCTGTTTCCGATGTACCTCCTGGTCACGTTG
CTGTTTCCGTCGGTGAGAATCGTCGGAGATACGTTGTCCGTGCTAAGCATTTGAACCATC
CGATTTTCCGACGGCTTCTAGCTGAGGCTGAGGAAGAGTATGGATTCGCTAACGTTGGTC
CGTTAGCGATTCCTTGTGATGAATCGTTGTTTGAGGATATTATTGCGATTGTGACTCGGT
GTGAGTCGTCATCATCATCTGGACGAGGTAATCCTCCGGCGGCGACGCTTGAGGATCTCC
GGAGGTGTAGTCATGTTGGATTGGCGAAGAACAACGTTGAATCTCGTCCGTTACTTCCTG
GGATTGCTGAAAAATCTGTGTGCTAATGAAATTTGCAAACGGAATGTGATTGAAACTTTG
GTTAGAGATTTAATTTTGAAAGTTTTAATTTAATTTCGTTGCTATTTTTTTTAATGATTT
TTGACTTTGTTTTTGGG

Last Updated on 06.12.2018 16:11