Sequences from confirmation amplicon


Confirmation sequences for line 973D11 and hit At3g13530 are shown.
Line ID 973D11
Locus At3g13530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4413707
Primers used for PCR 25hq - 8474, show primer details
   
Sequence Name 31-K112877-0022-973-D11C-8409
GenBank Accession LN500237 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112877-0022-973-D11C-8409
TTTTCTTTTCTTTGATCCTGCAAAAGAGGAAGTCTACTTTCATGTCTCTCTATGCCAGTG
ATATGCTTAACATAATCCAGTTGACCAGAATAATGTCTTGAAGGGGGTTCTTTATCCAAC
AAGCTAAGCAAAGGTCGAACTTGTTCTGTCTGAGTAACAGGAAAACCATTAGAGGCGCCC
TCTGCCAGCTTCTGAAGCTTATCTGTCGATGTCCTATTGGCGGAAAGAGATATTCGATGC
TGTTGTCTAACATCTTCTGTGCCAGAGGTTGAGGCATCTGGTGCAACACTGCTTACTCTA
GGCTTATCACCATCTGGGTGCAAAGCATCTGGTTGATGGACATCTGATCTTTGAGAATTT
GAGGTTGAAGCATGAGAAGGCTCTTCACCACCCCCATGCCTAGTTTTCAATACATCAGGC
TGATCAATCATACTGAGTGAAGAAGTCTCATTTTGACCAAAAATAGGATTGTTAGGGTCA
AGTTGACCAGAGCGCACTCGTGGAGCTTGACCATCCCGGCCCCGGGAGATAGAAGCCAGC
CGGGTT
   
Sequence Name 68-K112878-0022-973-D11C-25hq
GenBank Accession LN512047 [GenBank]
Sequencing primer gene specific primer, 25hq: 5'-CTTTATAGCTTGAATGAGGCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K112878-0022-973-D11C-25hq
GGGGCCTGGATGTCAGCTCCCGAGTGCGCTCTGGTCAACTTGACCCTAACAATCCTATTT
TTGGTCAAAATGAGACTTCTTCACTCAGTATGATTGATCAGCCTGATGTATTGAAAACTA
GGCATGGGGGTGGTGAAGAGCCTTCTCATGCTTCAACCTCAAATTCTCAAAGATCAGATG
TCCATCAACCAGATGCTTTGCACCCAGATGGTGATAAGCCTAGAGTAAGCAGTGTTGCAC
CAGATGCCTCAACCTCTGGCACAGAAGATGTTAGACAACAGCATCGAATATCTCTTTCCG
CCAATAGGACATCGACAGATAAGCTTCAGAAGCTGGCAGAGGGCGCCTCTAATGGTTTTC
CTGTTACTCAGACAGAACAAGTTCGACCTTTGCTTAGCTTGTTGGATAAAGAACCCCCTT
CAAGACATTATTCTGGTCAACTGGATTATGTTAAGCATATCACTGGCATAGAGAGACATG
AAAGTAGACTTCCTCTTTTGCATGGATCAAATGAAAAGAAAAACAACGGAGATCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTGTGATGGTCAATATGGAGAAAAAAAAAAA
ATAATTACCCCTTTTTTTTCAT

Last Updated on 06.12.2018 16:11