Sequences from confirmation amplicon


Confirmation sequences for line 973G12 and hit At1g05230 are shown.
Line ID 973G12
Locus At1g05230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1513597
Primers used for PCR 29hq - 8474, show primer details
   
Sequence Name 29-K112877-0022-973-G12C-8409
GenBank Accession LN499367 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K112877-0022-973-G12C-8409
ATTTACATGAAGCAGAGCCACATAGTCTGGATCACCTCCATTAAGCACTATGTTCATAGC
TACAATATCGACTGGAGCATAGATCACAAAGGAAGCTGTAGGATCAGTGCAGCTCTCTTG
TAGGATCAGCATATTGCTCTGGCTAGAGTTTGCACTCTGTGACATAAAGAAAGATTGAAA
ACTCTTAGCTAGGATCAGATGATTATAACAATAGTTCAAAACCCGGTGTAACTCACATTT
ACCCGAAGAAGAGAAACACAGTTTCCGGTATCCCTCCCGTTAGCAATATGTGCCATTTCT
TGCACAACTCCTCCATTAGACAGAATATCCCACTGCAAAATCTCACAAAAACATCAAAAC
TCTGTCCAAAACAG
   
Sequence Name 66-K112878-0022-973-G12C-29hq
GenBank Accession LN511645 [GenBank]
Sequencing primer gene specific primer, 29hq: 5'-TCTGTTTTGGACAGAGTTTTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K112878-0022-973-G12C-29hq
TGTGCAGAAATGGCACATATTGCTAACGGGAGGGATACCGGAAACTGTGTTTCTCTTCTT
CGGGTAAATGTGAGTTACACCNGGGTTTTGAACTATTGTTATAATCATCTGATCCTAGCT
AAGAGTTTTCAATCTTTCTTTATGTCACAGAGTGCAAACTCTAGCCAGAGCAATATGCTG
ATCCTACAAGAGAGCTGCACTGATCCTACAGCTTCCTTTGTGATCTATGCTCCAGTCGAT
ATTGTAGCTATGAACATAGTGCTTAATGGAGGTGATCCAGACTATGTGGCTCTGCTTCAT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
GATTATT

Last Updated on 06.12.2018 16:11