Sequences from confirmation amplicon


Confirmation sequences for line SALK_001056 and hit At4g12730 are shown.
Line ID SALK_001056
Locus At4g12730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7492296
Primers used for PCR 20ih - LBb3, show primer details
   
Sequence Name 12-K113191-SALK_001056C-20ih
GenBank Accession LN484616 [GenBank]
Sequencing primer gene specific primer, 20ih: 5'-TCTTACTCGACTTAGGAGCTGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K113191-SALK_001056C-20ih
GCACAACACTTTATCGATCTTGTAAACTATCAATGGCTCTTGATCCTTTAACGTCCCCAT
TACCTTCGCCGTCACAACGTCCGTCTCCAGCGTCACGTCTTCTCCGTCATTTTGAACGGT
GAAATCAAACTTGTTGTTACCTTCCGTCGCTAACGTGTTAACGGCGCCGTTACCTGATCT
AAGCATTTGTAACGACTGGTAAACCGGCATGCCGTGGTAAAGCACTAACGCAGTCTTGTT
CGCCGGAGAAAGGGATTTGAATTTCGGCATGAACTTACCGACAGCGCTGTCGGAGGGACA
GAACACCGTTAAGCCTCCGTCTACGGTATCTTGGAACGTTTTGTCGGCGCCGGTTGACTT
CAAGATGTCGGAGAAAGCTTTGCACCCTTGTTTTTCGAGGATTGTGGTGAGGATGAGGTC
GCTGGGACTCGCCGTGGGAGCTTCTGCTTCCGGCGAGGTTAAGACTTGGCTGATATATTG
TGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTTGATGGTGGTTCCG
   
Sequence Name 01-K113190-SALK_001056C-R210
GenBank Accession LN484283 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K113190-SALK_001056C-R210
GAATATATCCGGCAAGACTTAACCTCGCCGGAAGCAGAAGCTCCTTTGGCGAGTCCCAGC
GACCTCATCCTCACCATAATCCTCGAATATAAAGGGTGCAAAGCTTTCTCCGACATCTTG
AAATCAACCGGCGCCTACAAAACTTTCCAAGATACCGTAGACGGAGGCTTAACGGTGTTC
TGTCCCTCCGACAGCGCTGTCTGTAAGTTCATGCCGAAATTCAAATCCCTTTCTCCGGCG
AACAAGACTGCGTTAGTGCTTTACCACGGCATGCCGGTTTACCAGTCGTTACAAATGCTT
AAATCAGGTAACGGCGCCGTTAACACGTTAGCGACGGAAGGTAACAACAAGTTTGATTTC
ACCGTTCAAAATGACGGAGAAGACGTGACGCTGGAGACGGACGTTGTGACGGCGAAGGTA
ATGGGGACCTTAAAGGATCAAGAGCCATTGATAGTTTACAAGATCGATAAAGTGTTGTTG
CCTAGAGAAATCTACAAGGCCGTTAAGACGTCCGCTCCAGCTCCTAAGTCGAGTAA

Last Updated on 06.12.2018 16:11