Sequences from confirmation amplicon


Confirmation sequences for line SALK_007406c and hit At3g57670 are shown.
Line ID SALK_007406c
Locus At3g57670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21372890
Primers used for PCR 79sm - LBb3, show primer details
   
Sequence Name 40-K116586-SALK_007406C-79sm
GenBank Accession LN485298 [GenBank]
Sequencing primer gene specific primer, 79sm: 5'-CAAATAGAAATAAACCGTTGGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K116586-SALK_007406C-79sm
ATCAATTAGATGATATAGCTTTTAGTTTGAAAAGCCCTACCTCCATCAAAACTTGGTCCA
TAGATTTAGGTTTCTTATAATTAATATATATACCGAATTACTTAGTACTTATAGTAATAT
ATATAAGCTATTTAGACTGCATTGACTCATGATCATTGTCTAATTGTTCTACCTCAGAGG
CAGGCTCATCTTCTTCATCAAACCCATCAATTCCGTAGGCTCCATGACCATTCCCAAAAG
CCTTGATGTGATCTTTGAGAGATCTCTTGTGCTTGAAATCAGATCCACATATGCAATACC
AAAGTTTGCCACAATTCTTCTCATGTGTTCTCCAGTCCCCTCGGACTGCGAAAGCCTTTC
CACATTTCCTACACATGAAAGGTTTGATCCCATGTTTTCTCTTCAGACATTGACCATGTT
TTCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGG
   
Sequence Name 23-K116585-SALK_007406C-R210
GenBank Accession LN484955 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K116585-SALK_007406C-R210
AAAGGCTTTCGCAGTCCGAGGGGACCGGAGAACACATGAGAAGAATTCGTGGCAAACTTT
GGTATTCGCATATGTGGATCTGATTTCAAGCACAAGAGATCTCTCAAAGATCACATCAAG
GCTTTTGGGAAATTGGTCATGGAGCCTACGGAATTGATGGGTTTGATGAAGAAGATGAGC
CTGCCTCTGAGGTAGAACAATTAGACAATGATCATGAGTCAATGCAGTCTAAATAGCTTA
TATATATTACTATAAGTACTAAGTAATTCGGTATATATATTAATTATAAGAAACCTAAAT
CTATGGACCAAGTTTTGATGGAGGTAGGGCTTTTCAAACTAAAAGCTATATCATCTAATT
GATCATAGGAAAAAAATGAATCAAGAGCACTTGGAAAATTTTAAATTGTATCTTTAGCTT
CCTAGTTAAATTTATTGCAAGACAATGTAGCAGTCTAACCAATGAGGTTCCCAAC

Last Updated on 06.12.2018 16:11